生物技术通报Issue(10)：77-88,12.DOI:10.13560/j.cnki.biotech.bull.1985.2015.10.015
利用分子生物学技术提高微生物有机溶剂耐受性的研究进展
Research Advances on Molecular Techniques for Improving Microbial Tolerance to Organic Solvents
摘要
Abstract
Organic-solvent-tolerant microorganisms can survive and grow in the higher concentrations of organic solvents, which exhibit significant application advantages in the field of the non-aqueous phase biocatalysis. Compared to natural screening, long-term domestication and traditional mutagenesis methods, molecular technique is a more rational and efficient way to obtain organic-solvent-tolerant strains. This paper mainly summarizes the research advances on the improvement of microbial organic-solvent tolerance by the reformation of functional genes related to the resistance to organic solvents and transcriptional factors using molecular biology techniques, also prospects the application of organic-solvent-tolerant strain.关键词
有机溶剂耐受性/分子生物学技术/工程菌/生物转化Key words
organic solvent tolerance/molecular biology techniques/engineered strain/biotransformation引用本文复制引用
王艳霞,刘祥胜,王敏,骆健美..利用分子生物学技术提高微生物有机溶剂耐受性的研究进展[J].生物技术通报,2015,(10):77-88,12.基金项目
国家自然科学基金项目（21306138），天津市应用基础与前沿技术研究计划（自然科学基金）（13JCYBJC20600），天津科技大学大学生实验室创新基金项目 （）
