生物技术通报Issue(10)：111-118,8.DOI:10.13560/j.cnki.biotech.bull.1985.2015.10.019
干旱和低温胁迫影响烟草幼苗海藻糖代谢的差异比较
Comparison of Metabolic Differences of Trehalose inNicotiana tabacum Seedlings Under Drought and Chilling Stress
摘要
Abstract
In order to investigate differential response of trehalose metabolism to drought and low temperature stress, and roles of trehalose in the forming of drought and chilling resistance, tobacco seedlings(cv. Yunyan 203)were treated under drought and low temperature stresses. Then the changes of trehalose content, their activities of enzymes related to metabolism(TPS, TPP and THase)and gene expression were detected in the tobacco leaves during the drought and chilling stress at 4℃. The results indicated that trehalose content increased firstly, achieved the maximum level in 2 d, and the trehalose content in the leaves under drought stress always maintained higher level than that under the chilling stress. Similar to the change pattern of trehalose content, TPS, TPP activities were increased firstly and then decreased, and showed higher under the drought stress. On the other hand, THase activity demonstrated persistent increase under the drought and chilling stress, and was higher under chilling stress than that under drought stress. The expression level ofTPS,TPP, andTHasegenes was higher in the drought stress than the control at 0 d, but the expression level under chilling stress was lower than the control. Above results showed that drought and chilling stress induced the accumulation of trehalose, the drought stress led to relatively higher level of trehalose content, related enzyme activities and gene expression, implying that response of trehalose to drought stress was more sensitive than that to chilling stress.关键词
干旱胁迫/低温胁迫/海藻糖代谢/胁迫响应/烟草Key words
drought stress/chilling stress/trehalose metabolism/stress response/Nicotiana tabacum引用本文复制引用
张建波,王莎莎,郝大海,杨慧芹,马文广,高雪,崔明昆,龚明..干旱和低温胁迫影响烟草幼苗海藻糖代谢的差异比较[J].生物技术通报,2015,(10):111-118,8.基金项目
国家自然科学基金项目（31260064），中国烟草总公司云南省公司科技计划项目 （）
