生物技术通报Issue(10)：125-130,6.DOI:10.13560/j.cnki.biotech.bull.1985.2015.10.021
木薯MeASR基因克隆及表达分析
Clone and Expression ofMeASRGene in Cassava
摘要
Abstract
Abscisic acid-stress-ripening induced protein(ASR)plays an important role in response to abiotic stresses. In the present study we isolated a first ASR gene designatedMeASRfrom cassava. Sequence analysis showed that the open reading frame ofMeASR gene contained 330 bp, encoding 109 amino acids. Multiple sequence alignment and phylogenetic analysis indicated that MeASR protein contained the conserved domains as ASR family had, and had a close genetic relationship with SlASR4 of tomato. Subcellular location assay showed that MeASR protein was localized in nucleus. Real-time quantitative PCR assay revealed that expression ofMeASR was induced by osmotic stress and ABA treatments. These results suggested thatMeASRmight function as a transcription factor to involve in response to drought stress and ABA-signal regulation in cassava.关键词
木薯/MeASR基因/基因克隆/表达分析/亚细胞定位Key words
cassava/MeASR gene/gene clone/expression analysis/subcellular location引用本文复制引用
胡伟,颜彦,韦运谢,王文泉,夏志强,卢诚,侯晓婉,彭明..木薯MeASR基因克隆及表达分析[J].生物技术通报,2015,(10):125-130,6.基金项目
中央级公益性科研院所基本科研业务费（1630052014003，ITBB140204），海南省自然科学基金项目（314122），海南省重大科技专项 （）
