生物技术通报Issue(10)：165-170,6.DOI:10.13560/j.cnki.biotech.bull.1985.2015.10.026
柴胡内生真菌鉴定与抑菌活性筛选
Identification of Endophytic Fungi in Bupleurum and Screening of Antimicrobial Activities
摘要
Abstract
In order to determine the main distribution of endophytes and their broad-spectrum antimicrobial activity, 27 strains of endophytic fungi were isolated from the stems, roots and leaves of wild bupleurum at the Ganzi Tibetan Autonomous Prefecture of Sichuan Province. Identification of these strains was determined by their morphological features. They were classified as 9 fungal genera in which Aspergillus and Penicillium were the dominant. The antimicrobial activities from endophytic fungi were investigated by using the agar diffusion method and the cylinder plate method, andBacillus subtilis, Escherichia coli andStaphylococcus aureusas indicator microorganisms. The results showed that 26 strains, accounting for 96.30% of all the isolates, had antimicrobial activities to 2 indicators or 3. Endophytic fungi of CHG-01, CHG-04, CHJ-10, CHY-01, and CHY-03 had remarkable antimicrobial activities to 3 indicators respectively. There is natural antimicrobial substance in the secondary metabolites of endophytic fungi from the wild bupleurum, which provides a new source for searching novel antimicrobial products.关键词
内生真菌/抑菌活性/柴胡Key words
endophytic fungi/antimicrobial activity/bupleurum引用本文复制引用
李玲玲,罗合春,张先淑..柴胡内生真菌鉴定与抑菌活性筛选[J].生物技术通报,2015,(10):165-170,6.基金项目
重庆工贸职业技术学院院级项目 （）
