生物技术通报Issue(10)：171-176,6.DOI:10.13560/j.cnki.biotech.bull.1985.2015.10.027
产脂肪酶嗜酸性真菌的筛选、鉴定与产酶条件优化
Screening and Identification of Eosinophilic Fungal Strain Producing Lipase and Optimization of Its Lipase-producing Condition
摘要
Abstract
This experiment was devoted to screen the strains of high-yield lipase, identify the genera, and define the optimal fermentation conditions. The strains were preliminary screened from greasy soil nearby Lanzhou urban area through Victoria blue and rhodamine B plates. The strains were re-screened through a copper soap spectrophotography measuring lipase activity while using emulsified olive oil as the substrate, finally a fungal strain LZ-6 with high-yield lipase was obtained. It was identified asActinomucor elegans through morphological observation and molecular identification. The fermentation conditions for lipase production optimized by the single factor experiments and the orthogonal experiments with strain LZ-6 were:yeast powder 1%, olive oil 2%, 0.05% MgSO4·7H2O, 0.1%(NH4)2SO4, 0.2% K2HPO4, fermentation temperature at 30℃, pH5.0, 2% inoculums volume, and fermentation time of 5 days;under the optimal condition, the lipase activity of strain LZ-6 reached 13.35 U/mL, it increased 1.78 times as before optimization.关键词
真菌/脂肪酶/筛选/鉴定/优化Key words
fungi strain/lipase/screening/identification/optimization引用本文复制引用
赵军,赵淑琴,杨孝朴,刘晓丽..产脂肪酶嗜酸性真菌的筛选、鉴定与产酶条件优化[J].生物技术通报,2015,(10):171-176,6.基金项目
甘肃省财政厅项目 （）
