生物技术通报Issue(10)：184-190,7.DOI:10.13560/j.cnki.biotech.bull.1985.2015.10.029
同步脱氮除硫菌的筛选鉴定及其生长特性研究
Screening,Identification and Characterization of Strains Coupling Nitrogen Removal with Sulfide Removal
摘要
Abstract
In order to promote the engineering application of denitrification and desulfurization process, 2 highly-active autotrophic denitrifying bacterial strains H3 and H7 were screened from stable UASB with denitrification and desulfurization, and the strains were identified and characterized. Two strains were both gram negative bacteria, the 16S rDNA sequence analysis revealed that strain H3 and H7 had a similarity of 99.6%, 98.9% withPseudomonas stutzeriCONC12 andP. stutzeri19smn4 respectively. Combing the morphologic, physiobiochemical characteristics and the analysis of its 16S rDNA, 2 strains were identified asP. stutzeri. The results of growth and denitrification of 2 strains demonstrated that the optimal initial pH for H3 and H7 growth were 6.94 and 6.88 respectively, for denitrification were 6.77 and 6.56 respectively. The optimal temperatures for growth were 30.4℃ and 30.6℃, for denitrification were 31.4℃ and 31.2℃ respectively, both of H3 and H7 were non-resistant strains to salt.关键词
脱氮除硫菌/16S rDNA/反硝化特性/非耐盐菌株Key words
desulfurization denitrifying bacteria/16S rDNA/denitrification characteristic/non-resistant strain to salt引用本文复制引用
黄俊,张诗颖,王翻翻,李月寒,吴鹏,宋吟玲..同步脱氮除硫菌的筛选鉴定及其生长特性研究[J].生物技术通报,2015,(10):184-190,7.基金项目
江苏省高校自然科学基金项目（13KJB610014），江苏省环境科学与工程重点实验室开放基金项目（Zd131201），江苏高校优势学科建设工程资助项目 （）
