生物技术通报Issue(10)：230-235,6.DOI:10.13560/j.cnki.biotech.bull.1985.2015.10.001
东北林蛙发育早期TLR2的表达及免疫应答特征
The Expression and Immune Response Characteristics of TLR2 at the Early Development Stage ofRana chensinensis
摘要
Abstract
It was to study the expression and immune response characteristics of TLR2 at the early development stage ofRana chensinensis. Immunohistochemistry and RT-PCR were used to locate and quantify the TLR2 mRNA from stage 11 to stage 42 ofRana chensinensis. Tadpoles at stage 29 were injected with antigen LTA, LPS, ZymoA and Poly I∶C intraperitoneally. The expression changes of TLR2 mRNA were detected by RT-PCR within 24 h. TLR2 is widely distributed at the early development stage ofRana chensinensis. The relative expression of TLR2 mRNA increased form 0.187±0.021 to 0.373±0.031 at stage 25, and then returned to 0.170±0.020 progressively. The expression of TLR2 mRNA had no significant change in the LPS group and the Zymo A group(P>0.05). TLR2 mRNA of the LTE group peaked twice at 4 h与12 h after antigen injection(P<0.01);and the PolyI:C group peaked at 8 h(P<0.01). The expression character of TLR2 matches with its living environment at the early development stage ofRana chensinensis.TLR2 did not responses to the challenge of LPS and ZymoA timely at the stage 29, on the other hand, TLR2 recognized and responsed to LTA and PolyI∶C group effectively. TLR2 ofRana chensinensis possesses the recognition and response characteristics to PAMPs both of fish and mammals.关键词
东北林蛙/Toll样受体2/免疫应答/病原相关分子模式Key words
Rana chensinensis/TLR2/immune response/pathogen-associated molecular patterns引用本文复制引用
王泽,赫天一,陆宇燕..东北林蛙发育早期TLR2的表达及免疫应答特征[J].生物技术通报,2015,(10):230-235,6.基金项目
沈阳师范大学博士启动基金项目（054-55440107029），沈阳师范大学生态与环境研究中心主任基金项目 （）
