生物技术通报Issue(11)：153-158,6.DOI:10.13560/j.cnki.biotech.bull.1985.2015.11.020
甜瓜CmERFIII-1转录因子基因cDNA的克隆与表达分析
Cloning and Expression Analysis of the Ethylene-responsive Transcription Factor III-1 Gene cDNA from Melon(Cucumis melo)
摘要
Abstract
According to the clustering results of the ERF subfamily in melon,CmERFIII-1(accession ID:MELO3C005465)was chosen to be studied. A pair of specific primers was designed based on the cDNA ofCmERFIII-1. The full-length cDNA ofCmERFIII-1 was cloned by RT-PCR from the fruit of melon(Cucumis meloL.cv. Hetao).The length of cDNA was 711 bp, encoding 236 amino acids. Sequences analysis and the phylogenetic trees showed that the protein amino acid sequence ofCmERFIII-1 was closely related to two ERF subfamily member ofCucumis sativus(GeneBank ID:XP_004143677.1、XP_004158119.1). The Real-time quantitative RT-PCR assay showed CmERFIII-1genewasexpressed and its expression levels were different in tissues of the plant and fruits of different developmental stages . The expression level of this gene was highest in leaf.关键词
甜瓜/ERF/转录因子/表达分析Key words
melon/ERF/transcription factor/expression analysis引用本文复制引用
郭呈宇,孙晓蕾,邵琪,白立华,牛一丁,哈斯阿古拉..甜瓜CmERFIII-1转录因子基因cDNA的克隆与表达分析[J].生物技术通报,2015,(11):153-158,6.基金项目
国家自然科学基金资助项目 （）
