生物技术通报Issue(11)：179-185,7.DOI:10.13560/j.cnki.biotech.bull.1985.2015.11.023
毕赤酵母GS115中N-乙酰转移酶在大肠杆菌中的克隆表达与性质研究
Cloning,Expression and Characterization of Enzyme for Mpr1 from P. pastorisGS115 in RecombinantE.coli
朱海峰 1吴丹 2吴敬1
作者信息
- 1. 江南大学 食品科学与技术国家重点实验室，无锡 214122
- 2. 江南大学生物工程学院 工业生物技术教育部重点实验室江南大学，无锡 214122
- 折叠
摘要
Abstract
Due to the nature inP. pastorismethanol metabolism, it suffers much more ROS oxidative stress. There is one Mpr1 enzyme inP. pastoris. It plays significant physiological roles in ROS oxidative stress resistance ability and related research is still blank. For a detailed study about the physiological characteristics ofP. pastoris Mpr1, Mpr1 fromP. pastorisGS115 had been successfully expressed inE.coli JM109. Fermentation optimization of recombinant cell was studied from induction temperature, IPTG induction concentration, Initial induction OD by using Response Surface Analysis, activity reached(610.3±9.5)mU/mL. Enzymatic properties showed that the optimal pH of Mpr1 was about 7.0 to 7.5, the optimum temperature of Mpr1 was 30℃. In order to explore the nature of Mpr1, PQE30-E.coli JM109 strain and PQE30-Mpr1-E. coli JM109 strain were cultured under the same fermentation conditions in this experiment. The results showed that the growth capacity of the recombinant strain was stronger. The reason is that Mpr1 reduces levels of intracellular ROS.关键词
N-乙酰转移酶/大肠杆菌/毕赤酵母GS115/响应面分析法/发酵优化Key words
N-acetyl transferase/Escherichia coliJM109/P. pastoris GS115/response surface analysis/fermentation optimiza-tion引用本文复制引用
朱海峰,吴丹,吴敬..毕赤酵母GS115中N-乙酰转移酶在大肠杆菌中的克隆表达与性质研究[J].生物技术通报,2015,(11):179-185,7.
