生物技术通报Issue(11)：228-235,8.DOI:10.13560/j.cnki.biotech.bull.1985.2015.11.030
有机磷农药降解菌YC-YH1中mpd和ophc2的克隆及酶学性质分析
Cloning and Characterization ofmpd andophc2from an Organophosphorus Pesticide-degrading Bacteria YC-YH1
摘要
Abstract
We cloned two organophosphorus hydrolase genesmpd andophc2 from an organophosphorus pesticide-degrading bacteria Pseudomonas stutzeriYC-YH1. The two genes were inserted into the expression vector pET-32a and transformed intoE. coli BL21(DE3) respectively. We used Ni-affinity column to purify enzymes and then study the nature of the two enzymes. It was suggested that the optimal temperature of MPH is 40℃, and the MPH displays high activity in the pH range of 8 to 12. The optimal temperature of OPHC2 is 30℃. In the pH ranging of 8 to12, OPHC2 has higher activity. The mixture of MPH and OPHC2 in the proportion of 11 showsed higher activity in the range of 30℃ to 40℃, the enzyme showsed more than 95% activity in the range of pH8-12. A variety of metalions affect the activity of the enzymes and the enzymes are sensitive to SDS and EDTA, but the compound could reduce the sensitivity to the metalions.关键词
有机磷水解酶基因/MPH/OPHC2/纯化/酶学性质Key words
organophosphorus hydrolase gene/MPH/OPHC2/purification/enzymatic property引用本文复制引用
贾阳,史延华,任磊,乔铖,王俊欢,闫艳春..有机磷农药降解菌YC-YH1中mpd和ophc2的克隆及酶学性质分析[J].生物技术通报,2015,(11):228-235,8.基金项目
国家自然科学基金项目（31170119），中国农业科学院基础研究基金项目 （）
