生物技术通报Issue(11)：243-250,8.DOI:10.13560/j.cnki.biotech.bull.1985.2015.11.032
感染时间对MDCK细胞中H1N1甲型流感病毒扩增过程的影响
Effects of TOI on H1N1 Virus Propagation in MDCK Cell Culture
摘要
Abstract
In order to improve the production efficiency of influenza vaccine in processes based on MDCK cell culture, the effects of TOI on influenza A(H1N1)virus propagation in MDCK cells were investigated. By infecting MDCK cells with H1N1 influenza virus at different TOI, the characteristics of MDCK growth and metabolism and influenza A(H1N1)virus were studied. The results showed that maximum influenza A(H1N1)virus yield reached when TOI was 72 h. However, the cell specific virus yield was reduced as TOI increased. To find out the reason behind this phenomenon, further analysis of TOI and CCI effects on cell specific virus yield were studied. Results showed that, the phenomenon was caused by cell status rather than high cell density. The study reveals the importance of cell status difference due to TOI, showed that influenza A(H1N1)virus production efficiency in MDCK cells could be improved by cell state adjusting.关键词
感染时间/流感病毒/MDCK细胞Key words
TOI/influenza virus/MDCK cells引用本文复制引用
黄锭,刘旭平,范里,赵亮,谭文松,陈则..感染时间对MDCK细胞中H1N1甲型流感病毒扩增过程的影响[J].生物技术通报,2015,(11):243-250,8.基金项目
国家自然科学基金项目（21206040，21406066），国家“863”计划（2012AA02A303），国家重大专项（2013ZX10004003-003-003），中央高校基本科研业务费 （）
