生物信息学2015，Vol.13Issue(3)：170-178,9.DOI:10.3969/j.issn.1672-5565.2015.03.05
癌症DNA甲基化调控位点的识别
Identification of cancer DNA methylation regulatory sites
摘要
Abstract
DNA methylation is an important epigenetic modification,which plays an important role in the regulation of gene transcription.Abnormal DNA methylation may lead to cancer and disease,and identifying oncogene-related DNA methylation gene regulatory sites is important for the development of mechanisms to resolve the occurrence of cancer and identify new cancer markers. In this study,we integrate high-throughput DNA methylation profiling and gene expression profiling of pan-cancer genome in TCGA, then identify oncogene-related DNA methylation regulation sites.For each cancer,we calculate the correlation between methylation of CpG sites and gene expression, and filter the CpG sites, which regulate downstream genes( including strong regulatory sites, weak regulatory sites and not regulatory sites).The results show thatonly half of the CpG sites regulate the downstream genes.Analyzing of regulatory sites that is shared between cancers show that regulatory sites are not necessarily the same in different cancer,and the presence of cancer-specific methylation regulatory sites. Moreover,gene function enrichment analysis of differential DNA methylation and differentially expressed genes show that genes regulated by methylation are indeed involved in the development of cancer-related functions. The results of this study are an important supplementation to the current DNA methylation regulatory sites set, and an important resource to identify new molecular markers characteristics of cancer.关键词
DNA甲基化/基因表达/转录调控/癌症Key words
DNA methylation/Gene expression/Transcriptional regulation/Cancer分类
医药卫生引用本文复制引用
韦云真,刘晓娟,王芳,苏建忠,张岩,刘洪波..癌症DNA甲基化调控位点的识别[J].生物信息学,2015,13(3):170-178,9.基金项目
国家自然科学基金项目(61403112, 31371334). （61403112, 31371334）
