山西农业大学学报（自然科学版）2015，Vol.35Issue(6)：655-659,5.
碱性蛋白酶制备高水解度羊骨胶原肽的工艺研究
Preparation of Sheep Ossein Peptide with High Degree of Hydrolysis by Alcalase
摘要
Abstract
To improve the nutritional and physiological function of bone collagen ,parameters were optimized to prepare ossein peptides with high degree of hydrolysis (DH) by hydrolyzing sheep bone powder with the protease of alcalase . DH was determined by o-phthalaldehyde(OPA) methodology .In light of the results of single-factor experiments ,re-sponse surface analysis was carried out and a mathematical model was established to describe the DH in response to the levels of bone powder concentration (X1 ) ,temperature(X2 ) ,time(X3 ) ,and the amount of added alcalase .Variance a-nalysis suggested that the model showed high fitting degree .Sheep bone powder was hydrolyzed at pH 9 and under the adjusted optimized parameters of the model ,X1 、X2 、X3 、X4 was 9% (W/V)、45 ℃ 、325 min and 4.25% (E/S)respec-tively ,the determined DH was 28.12% ,which was close to the predicted 28.39% .This further confirmed the reliabili-ty of the established model which can be used to predict the DH when sheep bone powder was hydrolyzed by alcalase . The results of this study will also provide a theoretical guidance for the high-value and safe utilization of sheep bone .关键词
羊骨粉/胶原多肽/碱性蛋白酶/邻苯二甲醛法Key words
Sheep bone powder/Collagen polypeptide/Alcalase/O-phthalaldehyde methodology分类
轻工纺织引用本文复制引用
甄守艳,霍乃蕊,王凯,程稚玲,王钰慧,姚薇..碱性蛋白酶制备高水解度羊骨胶原肽的工艺研究[J].山西农业大学学报（自然科学版）,2015,35(6):655-659,5.基金项目
国家自然科学基金(31201347) （31201347）
山西省回国留学人员项目(2014-042) （2014-042）
