陕西师范大学学报(自然科学版)Issue(6):65-70,6.DOI:10.15983/j.cnki.jsnu.2015.06.365
PI3P 和 NO 在 UV-B 诱导蚕豆气孔关闭中的关系
The interactions of PI3P and NO in UV-B-induced stomatal closure of broad bean
摘要
Abstract
By stomatal bioassay and measurement of endogenous nitric oxide (NO)level in guard cells,the interactions of NO and phosphatidylinositol 3-phosphate (PI3P)[the product of phos-phatidylinositol 3-kinase (PI3K)]in UV-B-induced stomatal closure were studied in the epidermal strips of abaxial surface of broad bean(Vicia faba L.)leaves.The results showed that both the NO production in guard cells and stomatal closure induced by UV-B were significantly inhibited by PI3K inhibitors wortmannin (WM)and LY294002 (LY).Meanwhile,exogenous NO-relea-sing compound sodium nitroprusside (SNP)could completely reverse the inhibitory effect of LY and WM on the UV-B-induced stomatal closure,and WM and LY could not inhibit exogenous SNP-induced stomatal closure of broad bean.These results indicate that PI3P acts upstream of NO in the signal transduction pathway of UV-B-induced stomatal closure of broad bean.关键词
磷脂酰肌醇 3-磷酸/一氧化氮/紫外线 B 辐射/气孔关闭/蚕豆Key words
phosphatidylinositol 3-phosphate/nitric oxide/UV-B radiation/stomatal closure/broad bean分类
生物科学引用本文复制引用
樊彩明,王静,胡洁,贺军民..PI3P 和 NO 在 UV-B 诱导蚕豆气孔关闭中的关系[J].陕西师范大学学报(自然科学版),2015,(6):65-70,6.基金项目
国家自然科学基金(31170370);中央高校基本科研业务费专项资金 ()