山西医科大学学报2015，Vol.46Issue(10)：995-998,4.DOI:10.13753/j.issn.1007-6611.2015.10.011
毛细管电泳法测定螺内酯片剂中螺内酯的含量
Content of spironolactone in the spironolactone tablet determined by capillary electrophoresis
摘要
Abstract
Objective To establish a method for determining the content of spironolactone in spironolactone tablet by capillary electro-phoresis. Methods Capillary electrophoresis apparatus with diode array detector was adopted. The uncoated fused silica capillary (75μm × 57 cm, effective length of 10. 2 cm) was available. The pH 6. 5 phosphate buffer solution containing 20% acetonitrile and 0. 004 mmol/L cetyl trimethyl ammonium bromide( CTAB) were chosen as the background electrolyte, and the concentration of background e-lectrolyte was 0. 020 mol/L. The analysis voltage was set at 20 kV and the detection wavelength was set at 244 nm. Results There was a good linear relationship between the peak area and the concentration of spironolactone in the range of 1. 62-119. 9 μg/ml, and the linear regression equation was Y=877. 7X+4 238 (r2 =0. 991 6). The recovery rate was 91. 2%-106. 6%. The precision, sta-bility, repeatability of the method were good. Conclusion The developed method is simple, rapid and specific, which can be used for the determination of spironolactone in pharmaceutical preparation.关键词
螺内酯/毛细管电泳法/含量测定Key words
spironolactone/capillary electrophoresis/content determination分类
医药卫生引用本文复制引用
于海霞,李露,马慧芬,张红芬,陈安家..毛细管电泳法测定螺内酯片剂中螺内酯的含量[J].山西医科大学学报,2015,46(10):995-998,4.基金项目
山西医科大学创新基金资助项目( 01201305) （ 01201305）
