陕西医学杂志Issue(10)：1375-1377,3.DOI:10.3969/j.issn.1000-7377.2015.10.046
丙型肝炎病毒携带者病毒基因型分布特征及临床意义
Clinical significance and distribution characteristics of hepatitis C virus genotypes in hepatitis C virus carriers (Xi’an 710004)
李步荣 1张彤 1李丽华 1高宁 1甄长安 1张毅 1魏亚凤 1张西英1
作者信息
- 1. 西安交通大学第二附属医院检验科 西安710004
- 折叠
摘要
Abstract
Objective :To study the prevalence and distribution characteristics of hepatitis C virus (HCV) genotypes and to explore the clinic significance of HCV genotypes in HCV carriers .Methods :We conducted a cohort study with 90 participants including 58 male patients and 32 female patients .The HCV RNA levels of all sera sam‐ples were confirmed higher than 1 × 103 IU/ml .HCV genotyping was performed using type‐specific primers by means of polymerase chain reaction (PCR) and reverse dot blot (RDB) .The related data of all subjects were ana‐lyzed .GraphPad Prism 5 .0 software was used for statistical analysis .Results :The average concentration of HCV RNA in sera was 4 .94 ± 1 .36 log IU/ml .All 90 specimens were genotyped successfully .There were no significant difference between the different gender groups about the HCV RNA levels of all sera (P>0 .05) .HCV 1b was the most predominant genotype 48 .89% (44/90) ,followed by HCV 2a 42 .22% (38/90) .The single HCV genotype in‐fection was frequently detected among all patients .Its infection ratio was 95 .56% (86/90) .There was no significant difference between the different gender groups about the distribution of HCV genotypes(P>0 .05) .Conclusion :The single HCV genotype infection is primary infection style .HCV 1b is the most frequently detected genotype in pa‐tients infected with HCV ,following by HCV 2a .It is significant for clinical treatment to detect the genotypes of HCV .关键词
丙型肝炎病毒/基因型/聚合酶链式反应/反向斑点杂交Key words
Hepatitis C virus/Genotype/Polymerase chain reaction/Reverse dot blot分类
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李步荣,张彤,李丽华,高宁,甄长安,张毅,魏亚凤,张西英..丙型肝炎病毒携带者病毒基因型分布特征及临床意义[J].陕西医学杂志,2015,(10):1375-1377,3.
