陕西医学杂志Issue(11)：1450-1453,4.DOI:10.3969/j.issn.1000-7377.2015.11.003
外源性 Apelin-13对急性心肌梗死大鼠心肌细胞凋亡的影响研究∗
Effect of Apelin-13 on myocardial cell apoptosis induced by acute myocardial infarction in Rats
摘要
Abstract
Objective:To study the protection effect of Apelin-13 on myocardial cell of acute myocardial infarction in rats.Methods:The acute myocardial infarction methods (AMI)model was developed by ligation of the left anterior descending coronary artery.The surviving rats were divided into AMI group (AMI)and Apelin-13 trea-ted group (Apelin-13),and the groups of sham operation and Apelin-13 treated sham operation were also set up(n=8).After treatment for 14d,the rat hearts were taken,then detected the myocardial infarction area by TTC&Evans Blue staining and the pathological changes of cardiac tissue through HE staining.Furthermore,TUNEL method was used to detect the apoptotic myocardial cells,and the expression of the apoptotic protein Bcl-2,Bax and cleaved-Caspase-3 were detected by Western blotting.Results:Compared with the AMI group,after Apelin-13 treatment, the relative area of infarction myocardium in rats were significantly reduced,the accumulation of inflammatory cell in myocardial tissue and the number of apoptotic myocardial cells were decreased,the expression of pro-apoptotic pro-tein Bax and cleaved-Caspase-3 were increased and the expression of anti-apoptotic protein Bcl-2 was decreased.Con-clusion:Apelin-13 could effectively alleviate the acute myocardial infarction and myocardial cell apoptosis induced by myocardial ischemia,and Apelin-13 can be used as a drug for clinical treatment of acute myocardial infarction.关键词
心肌梗塞/病理生理学/Apelin-13/细胞凋亡/大鼠Key words
Myocardial infarction/physiopathology/Apelin-13/Apoptosis/Rats分类
医药卫生引用本文复制引用
张学民,冯丰,徐健,伍放..外源性 Apelin-13对急性心肌梗死大鼠心肌细胞凋亡的影响研究∗[J].陕西医学杂志,2015,(11):1450-1453,4.基金项目
沈阳市科技计划项目(F13-220-9-42) （F13-220-9-42）
