格尔德霉素体外抑制人肝癌细胞株HepG2增殖并促进凋亡OACSTPCD
Geldanamycin exhibits a significant antiproliferative effect and induces apoptosis in HepG2 cells
目的研究格尔德霉素对人肝癌HepG2细胞增殖及凋亡的影响。方法不同浓度格尔德霉素与人肝癌HepG2细胞共培养，采用MTT法检测增殖抑制率；采用流式细胞术Annexin V法检测细胞凋亡率。结果在0.2、0.4和0.8μmol/L浓度下，格尔德霉素均显著抑制HepG2细胞的增殖，在干预细胞48h后，其抑制率分别为20.36%、29.45%和42.52%，抑制率随药物浓度增加而升高；同时，细胞凋亡率分别为4.82%、24.47%和53.64%，也…查看全部>>
Objective To investigate the effects of geldanamycin on the proliferation and apoptosis in hu-man hepatoma cell lines HepG2. Methods HepG2 cells were incubated with different concentrations of gel-danamycin for 24 to 72 hours. Cell proliferation was determined by MTT assay. Cell apoptosis was detected by flow cytometry using annexin V and propidium iodide. Results Geldanamycin significantly inhibited the prolifera-tion of HepG2 cells at a dose-dependent mann…查看全部>>
刘明强;庄少鹉;刘丽莎
363000 福建省漳州市医院介入诊疗科363000 福建省漳州市医院介入诊疗科363000 福建省漳州市医院介入诊疗科
HepG2细胞格尔德霉素增殖调亡
HepG2 cellsGeldanamycinProliferationApoptosis
《实用肝脏病杂志》 2013 (4)
332-334,3
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