摘要
Abstract
Objective To investigate the changes of HLA-DR mRNA,and Th17 and CD4+CD25+Treg cells in peripheral blood mononuclear cells (PBMCs) in patients with liver failure. Methods HLA-DR mRNA was detected by PCR,and Th17 and CD4+CD25+Treg cells were measured by flow cytometry in 50 patients with liver failure,including 15 arisen from hepatitis B,12 from drug-induced liver injury (DILI),13 from alcoholic liver diseases,and 10 from autoimmune liver diseases,and in 17 patients with chronic hepatitis B and 10 healthy persons. Results The HLA-DR mRNA levels in PBMCs in patients with hepatitis B-induced liver failure was (134.5±15.2),much higher than (17.9±1.2)in DILI- ,(19.6±2.0)in alcoholic and [(11.2±1.2),P<0.05] in autoimmune liver disease-induced liver failure;the percentages of Th17 cells and CD4+CD25+Treg cells [(4.4±0.6)%and(3.9±0.6)% or less,respectively] in patients with different etiologic liver failures were not significantly different (P>0.05),while there were a significant difference as compared to (3.7±0.2)% and (6.1±0.4)% in patients with chronic hepatitis B or(2.1±0.7)% and(7.0±0.9)% in normal control (P<0.05);the percentages of CD4+CD25+Treg cells in 19 died patients decreased gradually,while it increased gradually in 31 survived patients. Conclusion The HLA-DR mRNA levels and CD4+CD25+Treg cells are closely related to the illness severity of patients with liver failure,and they may be used as parameters to estimate the clinical prognosis.关键词
肝衰竭/HLA-DR/Th17细胞/CD4+CD25+Treg细胞Key words
Liver failure/HLA-DR/Th17 cells/CD4+CD25+Treg cells
