右美托咪定对人中性粒细胞凋亡和呼吸爆发功能的影响OA北大核心CSTPCD
Effect of dexmedetomidine on apoptosis and respiratory burst of polymorphonuclear neutrophils in vitro
目的：观察右美托咪定（Dex）对离体培养的人中性粒细胞（PMN）凋亡率和呼吸爆发功能的影响。方法：取健康人外周静脉血，血浆－Percoll不连续密度梯度离心法分离出PMN，分为对照组，1 ng／mL Dex组，10 ng／mL Dex组和100 ng／mL Dex组，离体培养24 h ，取培养2 h和24 h的 PMN 悬液，检测 caspase-3活性、PMN 凋亡率和呼吸爆发功能。结果：与对照组比较3种不同浓度的Dex与PMN孵育2 h，PM…查看全部>>
Objective To investigate the effect of dexmedetomidine (Dex) on the apoptosis and respiratory burst of human polymorphonuclear neutrophils (PMN) in vitro. Methods The peripheral venous blood was collected from healthy volunteers. Isolation of PMN was performed by using the discontinuous plasma-Percoll gradient technique. PMN was randomLy divided into four groups: control group and three different concentrations of Dex (1 ng/mL,10 ng/mL,100 ng/mL) groups. PMN…查看全部>>
吴新海;邓若熹;万帆
518036 北京大学深圳医院麻醉科518036 北京大学深圳医院麻醉科518036 北京大学深圳医院麻醉科
右美托咪定中性粒细胞凋亡呼吸爆发
DexmedetomidinePolymorphonuclear neutrophilsApoptosisRespiratory burst
《实用医学杂志》 2015 (17)
2883-2886,4
广东省自然科研基金（编号s2013010011539）
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