实用医学杂志Issue(19)：3129-3132,4.DOI:10.3969/j.issn.1006-5725.2015.19.004
γ－干扰素对支气管哮喘小鼠气道炎症和分泌黏蛋白MUC5ac的影响
Effects of IFN-γ on airway inflammation and protein MUC5ac mRNA expression in mice with asthma
摘要
Abstract
Objective To investigate the effects of IFN-γon airway inflammation and protein MUC5ac mRNA expression in mice with asthma. Methods Forty c57bL/6 mice were randomly divided into control group, model group, IFN-γ 50,000IU group and IFN-γ 100,000IU group, 10 for each group. The change rate of airway resistance was detected after acetylcholine chloride provocation. The count of inflammatory cells in BALF was analyzed. Levels of IL-13 in BALF were measured by ELISA. The lung tissue sections were stained with Ab-PAS for identification of goblet cells proliferation. Expressions of MUC5ac mRNA in the lung tissues were determined by real time-PCR. Results As compared with the control group , the change rate of airway resistance , count of inflammatory cells , levels of IL-13 and MUC5ac mRNA in lung were significantly increased in the model group (P < 0.05). IFN-γ reduced airway resistance, inflammatory cells, levels of IL-13 and MUC5ac mRNA in the lungs (P < 0.05). Conclusions IFN-γ can regulate the balance of Th1/Th2, inhibit airway goblet cell proliferation , and protein MUC5ac mRNA expression in mice withasthma.关键词
哮喘/γ-干扰素/杯状细胞/白细胞介素-13/MUC5acKey words
Asthma/IFN-γ/Goblet cells/IL-13/MUC5ac引用本文复制引用
尹德锋,金明哲,王宋平,熊瑛..γ－干扰素对支气管哮喘小鼠气道炎症和分泌黏蛋白MUC5ac的影响[J].实用医学杂志,2015,(19):3129-3132,4.基金项目
四川省卫生厅科研课题资助项目 （）
