实用医学杂志Issue(20)：3291-3294,4.DOI:10.3969/j.issn.1006-5725.2015.20.002
张应力下大鼠骨髓间充质细胞成骨分化过程中硫化氢信号系统的作用
The role of the hydrogen sulfide signal system in rat bone marrow-derived mesenchymal cells osteogenesis under tensile stress
摘要
Abstract
Objective To evaluate the impact of hydrogen sulfide signal system during the process of rat bone marrow-derived mesenchymal cells osteogenesis under tensile stress. Methods After the H2S signal system of cell rooms regulated by tool drugs (Group A with propargylglycine;Goup B with PBS;Group C with GYY3147), 4 000 μ strain tensile stress were applied on rat BMMCs by four-point bending apparatus for 60 minutes. Four hours later, the H2S signal system and cystathionine-γ-lyase were detected. Meanwhile, the change of the alkaline phosphate, osteocalcin, procollagen typeⅠN-terminal propeptide, and runt-related transcription factor 2 mRNA level were also examined to evaluate the osteogenic ability. Results With the increase of H2S expression, the osteogenic capacity gradually increased, while the osteogenic capacity was compromised after the endogenous cystathionine-γ-lyase was inhibited. Conclusion The H2S signal system plays an important role during BMMCs osteogensis under tensile stress. The up-regulation expression of H2S may promote osteogenesis during distraction osteogenesis.关键词
张应力/骨髓间充质干细胞/硫化氢Key words
Tensile stress/Bone marrow-derived mesenchymal cells/Hydrogen sulfide引用本文复制引用
蒋校文,张翼,范晓升,邓轩..张应力下大鼠骨髓间充质细胞成骨分化过程中硫化氢信号系统的作用[J].实用医学杂志,2015,(20):3291-3294,4.基金项目
国家自然基金（编号81301651）；郴州市科技局项目 （）
