特产研究Issue(3)：22-26,5.
龙胆属6种药用植物的 ITS 2序列分析
ITS2 Sequence Analysis of Six Medicinal Plants in Gentiana L .
摘要
Abstract
To provide evidence for the molecular identification and phylogeny relationship analysis of six medicinal plants in Gentiana L .,three ITS2 sequence samples of three species in Gentiana L .were PCR amplified and sequenced ,and 27 samples from the GenBank were download-ed .Sequence features were compared and analyzed using ClustalX ,GeneDoc and MEGA 5.0 softwares ,and phylogenetic analyses were used to investigate their evolutionary relationships by neighbour -joining(NJ) ,maximum parsimony(MP) and maximum -likelihood(ML) .The results showed that there were 187 conserved sites and 39 variable sites in the ITS2 region of the six medicinal plants ;The G+C contents of ITS2 from G .scabra ,G .triflora ,G .manshurica ,G .rigescens were higher (60.2% ,60.6% ,60.6% ,and 60.0% ,respectively ) compared to those from G .macrophylla ,and G .straminea(58.2% and 56.3% ,respectively);among the six medicinal plants ,the genetic distances of three species between G .scabra ,G .triflora and G .manshurica were very small ,especially between G .scabra and G .manshurica ,which were only 0.005 , while the largest genetic distances between G .rigescens and G .straminea were 0.117;Medical plant species from section Pneumonanthe (G . scabra ,G .triflora ,G .manshurica) were proved to be closely related to that of section Cruciata(G .macrophylla ,G .straminea) ,and there was a distant relationship between G .rigescens and each of the other five medicinal plants in Gentiana L .based on phylogenetic analysis .The ITS2 sequence provides evidence for phylogeny relationship analysis of six medicinal plants in Gentiana L .关键词
龙胆属/ITS2序列/药用植物/亲缘关系分析Key words
Gentiana/ITS2/medicinal plants/phylogeny relationship分类
生物科学引用本文复制引用
王秋霞,孙海,魏云洁,张舒娜,杨振,张亚玉..龙胆属6种药用植物的 ITS 2序列分析[J].特产研究,2015,(3):22-26,5.基金项目
吉林省科技厅项目 （）
