生态环境学报Issue(7)：1124-1128,5.
假高粱根系分泌物对土壤细菌群落多样性的影响
Effects of root exudates from Johnsongrass on soil bacteria community diversity
摘要
Abstract
The aim of the present study is to investigate the effects of invasive plant Johnsongrass (Sorghum halepense (L.) Pers) root exudates on soil bacteria. In a greenhouse experiments, activated carbon was used to investigate root exudates of Johnsongrass because it adsorbs and thereby neutralizes root exudates. The experiment consisted of two combined soil treatments:with or without activated carbon. 16S-rDNA-V3-fragnent-base DGGE (denaturing gradient gel electrophoresis) was applied to explore variation of the soil bacterial structure. To calculate the diversity parameter, Shannon algorithm was used. The results showed that activated carbon led to a considerable decrease in the soil bacterial diversity level and abundance compared to the without activated carbon treatments. The Shannon diversity index is 2.54 and 3.24 separately in the 160 days. This indicates that Johnsongrass root exudates alter community structure and increase diversity of soil bacteria in rhizobacterial communities. It is different in the bacterial structure with the growth of the plant. The bacterial community showed a high difference with the 50%Jaccard's index of similarity in the florescence, suggesting that increases in root exudates support an increased soil bacterial population. DGGE analysis results show that Johnsongrass root exudates promoted some kinds of rhizobacterial, and show inhibition to some other kinds of the communities. This study establishes root exudates as a mechanism through which a plant is able to regulate soil bacterial community composition, and could be related to the invasive of exotic invasive plants and the soil microbes in rhizosphere.关键词
假高粱/根系分泌物/土壤细菌/DGGEKey words
Johnsongrass/root exudates/soil bacteria/DGGE分类
农业科技引用本文复制引用
刘纯,黄红娟,张朝贤,王茂云,陈小奇,王金信..假高粱根系分泌物对土壤细菌群落多样性的影响[J].生态环境学报,2013,(7):1124-1128,5.基金项目
国家自然科学基金 （）
