生态环境学报Issue(9)：1560-1569,10.DOI:10.16258/j.cnki.1674-5906.2015.09.021
微生物修复石油污染土壤的生态毒性指示
Study on Combined Bioindicators in Ecotoxicity Monitoring of Oil-contaminated Soil during Bioremediation
摘要
Abstract
Bacterial luminescence, content of photosynthetic pigments together with soil enzyme activity could be used to comprehensively reflecting the soil health condition. In order to explore the soil ecotoxicity patterns and biological indicator effects, bioremediation of oil-contaminated soil were conducted. Oil degrading bacterial consortium were built with three strains isolated from oil-contaminated soil. Bacterial luminescence, chlorophyll a and carotene contents, and soil catalase activity was employed to evaluate the ecotoxicity of soil sampled in different bioremediation phases. Good consistency can be obtained among phytotoxicity tests, soil enzyme activity and Photobacterium phosphoreum ecotoxicity tests. The results showed that the application of a mixed bacterial consortium was illustrated to effectively remediate oil-contaminated soil due to the high TPH removal efficiency, which reduced the crude oil concentration from 5 000 mg·kg-1 soil to 1 781 mg·kg-1 in only 40 d. The maximum inhibition of bacterial luminescence for Photobacterium phosphoreum in the dichloromethane/dimethyl sulfoxide extracts was observed at the initial stage of bioremediation and gradually dropped to normal. Compared with the control group, the chlorophyll a content of Triticum acstivnm L. was significantly inhibited in the different phases of bioremediation. In soils S1, the chlorophyll a content decreased by 39.3%to (1.36±0.04) mg·g-1. A significant negative correlation can be found between soil catalase activity and the residue of petroleum hydrocarbon. The correlation coefficient is-0.973. Soil extract on the 8th day of the bioremediation remained constant at a relative luminosity of 18.1%, with toxicity equivalent to that of 0.187 mg·L-1 HgCl2. Therefore, bacterial luminescence, phytotoxicity (inhibition of chlorophyll a and carotene contents), and soil catalase activity could potentially be sensitive indicators to evaluate the effectiveness of bioremediation techniques.关键词
石油污染土壤/生物修复/发光菌/生物指示Key words
oil contaminated soil/bioremediation/Photobacterium phosphoreum/combined-bioindicators分类
资源环境引用本文复制引用
沈伟航,朱能武,尹富华,王华金,党志..微生物修复石油污染土壤的生态毒性指示[J].生态环境学报,2015,(9):1560-1569,10.基金项目
广东省自然科学基金团队项目（9351064101000001）；教育部新世纪优秀人才支持计划项目 （）
