物理学报Issue(9)：260-268,9.DOI:10.7498/aps.62.094218
微生物远红外波段复折射率测定及模型构建*
Determination and model construction of microbes’ complex refractive index in far infrared band∗
摘要
Abstract
Bacillus subtilis, Aspergillus niger spore and Aspergillus niger mycelium are chosen for samples preparation. The above three samples’ reflection spectra in 2.5—15 µm band are recorded by using infrared microscopic spectrometer. From Kramers-Kronig (K-K) relationship, microbe samples’ complex refractive indexes in 6—14 µm band are calculated. According to the microbe samples’ absorption spectra, their water contents are calculated using the absorption characteristics of water, protein and nucleic acid. Based on the above three microbe samples’ water contents and complex refractive indexes in far infrared band, biologic particle’s complex refractive index model in 6—14 µm band are constructed and the model’s reliability is analyzed. The construction of biologic particle’s complex refractive index model has important significance in the development of rapid analysis and identification method of biologic samples, and the detection and identification technologies of biologic aerosol.关键词
光学参数/微生物复折射率模型/远红外波段Key words
optical parameters/complex refractive index of microbe/far infrared band引用本文复制引用
孙杜娟,胡以华,顾有林,王勇,李乐..微生物远红外波段复折射率测定及模型构建*[J].物理学报,2013,(9):260-268,9.基金项目
国家重点基础研究发展计划(973计划)(批准号:2011CB707900),国家自然科学基金(批准号:81127901,11161120324,11174141,11104140),江苏省自然科学基金(批准号:BE2011110, BK2011543)资助的课题.*Project supported by the National Basic Research Program of China (Grant No.2011CB707900), the from National Natural Science Foundation of China (Grant Nos.81127901,11161120324,11174141,11104140), and the Natural Science Foundation of Jiangsu Province of China (Grant Nos. BE2011110, BK2011543) （973计划）
