物理学报Issue(15)：1-7,7.DOI:10.7498/aps.62.154206
用于大景深三维纳米分辨多分子追踪的衍射光学元件的设计制备和实验研究*
Design, fabrication, and experimental demonstration of a diffractive optical element with long depth of field for nanoscale three-dimensional multi-molecule tracking*
摘要
Abstract
The development of real-time single-molecule detection and tracking technology in time and space for multiple bio-molecules in intact cells has important significance for the study on molecular behaviors in life processes. However, limited depth of field is the main drawback of conventional microscopy that prevents observation and tracking of multiple molecules in intact cells. Based on the principle of wavefront coding, the design and fabrication of a novel diffractive optical element (DOE) which combines a distorted diffraction grating with a double-helix point spread function (PSF) phase plate so that it can simultaneously perform multi-plane imaging with double-helix PSF and realize an extended depth of field is presented. Experiments have been carried out on a self-built microscopic system based on the novel DOE, showing that a depth of field can be up to 12 µm. Experimental results are in good agreement with the theoretical predictions, thus proving the feasibility of this method.关键词
多分子追踪/衍射光学元件/变形光栅/双螺旋点扩散函数Key words
multi-molecule tracking/diffractive optical element/distorted diffraction grating/double helix point spread function引用本文复制引用
于斌,李恒,陈丹妮,牛憨笨..用于大景深三维纳米分辨多分子追踪的衍射光学元件的设计制备和实验研究*[J].物理学报,2013,(15):1-7,7.基金项目
国家重点基础研究发展计划(973计划)(批准号:2012CB825802)、国家自然科学基金(批准号:61178080,61235012,11004136,60878053)、国家重大科学仪器设备开发专项(批准号:2012YQ15009203)和深圳市科技计划项目(批准号：JCYJ20120613173049560, GJHS20120621155433884, ZYC201105170233A)资助的课题.* Project supported by the National Basic Research Program of China (Grant No.2012CB825802), the National Natural Science Foundation of China(Grant Nos.61178080,61235012,11004136,60878053), the Special Funds of the Major Scientific Instruments Equipment Development of China (Grant No.2012YQ15009203), and the Science and Technology Planning Project of Shenzhen, China (Grant Nos. JCYJ20120613173049560, GJHS20120621155433884, ZYC201105170233A) （973计划）
