物理学报Issue(19)：1-7,7.DOI:10.7498/aps.64.190702
基于激光诱导叶绿素荧光寿命成像技术的植物荧光特性研究∗
Study of plant fluorescence prop erties based on laser-induced chlorophyll fluorescence lifetime imaging technology
摘要
Abstract
Plant fluorescence is a susceptible signal in plant fluorescence remote sensing detection. In order to solve this problem, a technique for plant chlorophyll fluorescence lifetime imaging is presented to evaluate living status for plant growth and environmental monitoring. A concave lens is used to expand laser beam at a wavelength of 355 nm, and the living plant is exposed in this laser light source to excite chlorophyll fluorescence. And the chlorophyll fluorescence signals are detected by an intensification charge coupled device. Time resolved measurement method is used in this article, so that every time the same fluorescence signals can be excited by the same laser pulse. Meanwhile, the delay time needed for triggering intensification charge coupled device should be changed consecutively, and the whole discrete fluorescence signal can be obtained. The discrete fluorescence signals from the particular location points of the plant are fitted. An improved method of forward iterative deconvolution is used to retrieve the corresponding fluorescence lifetime, and the high-precision fluorescence lifetime can be obtained. Furthermore, the fluorescence lifetime values at all the location points are retrieved to obtain the distribution map of chlorophyll fluorescence lifetime. This method can give the chlorophyll fluorescence image efficiently. The distribution map of fluorescence lifetime can more effectively reflect the plant chlorophyll concentration than the fluorescence intensity image does. The physical property of chlorophyll fluorescence lifetime from living plants has been studied preliminarily, indicating that the plant physiological status is related to its fluorescence lifetime to a certain extent; and the chlorophyll fluorescence lifetime and plant environment have a subtle and complex correlation. In the future, the relationship between chlorophyll fluorescence lifetime and plant environment will be expected to study with the cooperation of biophysicist.关键词
荧光寿命成像/激光诱导荧光/荧光雷达/叶绿素Key words
fluorescence lifetime imaging/laser-induced fluorescence/fluorescence lidar/chlorophyll引用本文复制引用
万文博,华灯鑫,乐静,闫哲,周春艳..基于激光诱导叶绿素荧光寿命成像技术的植物荧光特性研究∗[J].物理学报,2015,(19):1-7,7.基金项目
国家自然科学基金(批准号:61275185)资助的课题.@@@@* Project supported by the National Natural Science Foundation of China (Grant No.61275185) （批准号:61275185）
