西安交通大学学报（医学版）Issue(6)：758-764,781,8.DOI:10.7652/jdyxb201506009
肠道菌群失调对内脏敏感性的影响及其机制
The influence of intestinal dysbacteriosis on visceral sensitivity and possible mechanisms
邵珲 1王进海 1张杰 2杨龙宝 1谢丹红1
作者信息
- 1. 西安交通大学医学部第二附属医院 消化内科，陕西省肠胃动力疾病研究重点实验室，陕西省胃肠疾病临床研究中心，陕西西安 710004
- 2. 西安交通大学医学部第二附属医院 核医学科，陕西西安 710004
- 折叠
摘要
Abstract
Objective To detect the changes of visceral sensitivity in rats presenting intestinal dysbacteriosis and the expressions of tight junction protein (ZO-1)and Toll-like receptor 4 (TLR4)so as to explore the effect of intestinal dysbacteriosis on visceral sensitivity and the possible mechanisms.Methods We randomly divided 30 male SD rats of SPF grade into normal control group (n = 12 )and dysbacteriosis group (n = 18 ).Rats in dysbacteriosis group were administered with lincomycin hydrochloride (300 mg/mL),1 mL each time per rat once a day for 7 consecutive days;those in normal control group were fed with the same amount of saline.On the eighth day,six rats were randomly selected from normal control group and dysbacteriosis group respectively to detect whether the model was successful.After the model was successfully constructed,the remaining 12 dysbacteriosis rats were randomly divided into the negative control group and the probiotics intervention group with 6 in each.Rats in the intervention group were given probiotic bifidobacterium triple viable capsules (Bifico)orally,one capsule with 1/3 mL of saline,1 mL each time per rat once a day for 7 consecutive days;those in the negative control group received the same amount of saline.On the eighth day,fresh feces was cultured for flora to detect visceral sensitivity by abdominal withdrawal reflex (AWR),the mRNA and protein expressions of ZO-1 and TLR4 in the colon,and the expression of serum inflammatory cytokines IL-10 and TNFα.Results The expression of ZO-1 in the colon was significantly lower in the rats of dysbacteriosis group than those in the control group,and the expression of TLR4 was also significantly increased.Correspondingly,the expression of pro-inflammatory factor TNFα in the serum of the rats in dysbacteriosis group was significantly increased,while that of anti-inflammatory factor IL-10 was significantly lower than in the control group (P <0.05).Furthermore,compared with dysbacteriosis group,the expression of ZO-1 was increased significantly and TLR4 was decreased in probiotics group in varying degrees. Similarly,the expression of TNFα was obviously lower while that of IL-10 in the serum was higher (P < 0.05 ). Conclusion Inhibiting the expression of ZO-1 and increasing the expression of TLR4,thus leading to chronic low-grade inflammation, may be one mechanism of visceral hypersensitivity caused by intestinal dysbacteriosis. Probiotics may restore the dysbacteriosis and thus improve visceral hypersensitivity.关键词
肠道菌群失调/内脏高敏感/肠易激综合征/低度炎症/紧密连接蛋白(ZO-1)/Toll 样 4 受体(TLR4)/益生菌Key words
intestinal dysbacteriosis/visceral hypersensitivity/irritable bowel syndrome/low-grade inflamma-tion/tight junction protein (ZO-1)/Toll-like receptor 4 (TLR4)/probiotics分类
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邵珲,王进海,张杰,杨龙宝,谢丹红..肠道菌群失调对内脏敏感性的影响及其机制[J].西安交通大学学报（医学版）,2015,(6):758-764,781,8.
