西安交通大学学报（医学版）Issue(6)：829-835,7.DOI:10.7652/jdyxb201506023
MCM5和 P16INK4A在宫颈鳞癌组织中的表达及其临床意义
Expressions of MCM5 and P16INK4A in cervical squamous cell carcinoma
刘丽娜 1李瑶 2杨永秀 1王慧君 1赵静妮3
作者信息
- 1. 甘肃省妇幼保健院妇产科，甘肃兰州 730050
- 2. 兰州大学第一临床医学院妇产科学，甘肃兰州 730000
- 3. 兰州大学第一医院妇产科，甘肃兰州 730000
- 折叠
摘要
Abstract
Objective To investigate the expressions of minichromosome maintenance protein 5 (MCM5)and P1 6 INK4A gene and protein in cervical squamous cell cancer (SCC),cervical intraepithelial neoplasia (CIN)and normal tissues.Methods Real-time PCR and immunohistochemistry were used respectively to detect the expression levels of mRNA and protein of MCM5 and P1 6 INK4A in 40 cases of cervical cancer,1 1 cases of CIN I,1 5 cases of CIN Ⅱ - Ⅲ and 1 5 cases of normal cervical tissues.Results ① The expression levels of mRNA and protein of MCM5 and P1 6 INK4A in tissues of cervical squamous cell cancer were significantly higher than those in normal cervical tissues,CIN I and CIN Ⅱ-Ⅲ tissues (P <0.05).The expression level of P1 6 INK4A was significantly higher in tissues of cervical squamous cell cancer than in normal cervical tissues and CIN I tissues (P < 0.01 ). However,it did not differ from that in CIN Ⅱ-Ⅲ tissues (P >0.05 ).② The high expression of MCM5 mRNA and protein in cervical squamous cell carcinoma was positively correlated with clinical stage and differentiation grade of cervical cancer (P <0.01),but not correlated with age (P >0.05).P1 6 INK4A expression exhibited no correlation with clinical stage or age of patients (P >0.05),but positive correlation with differentiation grade (P <0.01).③ A positive correlation was found between MCM5 and P1 6 INK4A expressions in cervical cancer (r = 0.538,P <0.01).Conclusion The over-expressions of MCM5 and P1 6 INK4A may play an important role in the carcinogenesis and progression of cervical cancer.MCM5 may be used as a new marker of the proliferation of cervical cancer. Detection of P1 6 INK4A and MCM5 is of great significance to studying the grade and outcome of CIN,which may improve the rate of cervical cancer screening.关键词
微小染色体维持蛋白 5(MCM5)/P1 6 INK4A/宫颈鳞癌/免疫组化/实时荧光定量 PCRKey words
minichromosom maintenance protein 5 (MCM5)/P1 6 INK4A/cervical cancer/immunohistochemis-try/real-time PCR分类
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刘丽娜,李瑶,杨永秀,王慧君,赵静妮..MCM5和 P16INK4A在宫颈鳞癌组织中的表达及其临床意义[J].西安交通大学学报（医学版）,2015,(6):829-835,7.
