西安交通大学学报（医学版）Issue(6)：836-839,4.DOI:10.7652/jdyxb201506024
西安市采暖季与非采暖季 PM2.5染毒的人胚肺细胞基因差异表达研究
Genes’differential expression with PM2.5 exposure in human embryo lung cells between heating season and un-heating season in Xi’an City
摘要
Abstract
Objective To investigate the pathogen-related genes of atmospheric polluting disease so as to clarify the biology mechanism and provide the scientific basis.Methods By using the technique of dot blot hybridization,we analyzed genes’differential expression with cloning by exposure to ≥75 μg/m3 PM2.5 in heating season and < 75 μg/m3 PM2.5 in un-heating season in WI-38 human embryo lung cells.The levels of cytokines TNF-α,IL-2, IL-6 and IL-8 were determined by radio immunity assay. Results After 24h of treatment, compared with control group,more than 100μg/mL PM2.5 significantly increased TNF-α,IL-6 and IL-8 levels,and decreased IL-2 in WI-38 human embryo lung cells (P < 0.05 ).The clear stripe was found in 350 bp in 48 gene samples with segment with differential expression of genes exposed to different concentrations of PM2.5 in WI-38 human embryo lung cells.Through the dot blot hybridization,black brown spots were found in 41 samples in Tester cDNA hybridization,and no similar spots were found in all of the same samples in Driver cDNA hybridization. Conclusion PM2.5 exposure may induce the inflammatory damage of WI-38 human embryo lung cells.Obvious genetic damage was observed in those cells exposed to ≥75 μg/m3 PM2.5 in heating season.关键词
PM2.5/WI-38 人胚肺细胞/基因差异表达/斑点杂交/TNF-α/IL-2/IL-6/IL-8Key words
WORDS:PM2.5/WI-38 human embryo lung cell/differential expression of gene/dot blot hybridization/TNF-α/IL-2/IL-6/IL-8分类
医药卫生引用本文复制引用
杨宇轩,闫思琪,衣颖杰,张喆,聂雨晨,郭坤,于燕..西安市采暖季与非采暖季 PM2.5染毒的人胚肺细胞基因差异表达研究[J].西安交通大学学报（医学版）,2015,(6):836-839,4.基金项目
2013年国家级大学生创新训练项目(No.201310698066) Supported by the fund for The National-level University Students’Innovation Training Project (No.201310698066) （No.201310698066）
