西安交通大学学报（医学版）Issue(6)：840-844,861,6.DOI:10.7652/jdyxb201506025
β-榄香烯对人胃癌细胞 SGC7901作用的蛋白质组学研究
A proteomic analysis of effects ofβ-elemene on human gastric cancer cell line SGC7901
摘要
Abstract
Objective To investigate the effect of β-elemene on SGC7901 gastric cancer cell line and the potential proteins involved. Methods Human SGC7901 gastric cancer cells were treated with different concentrations ofβ-elemene.Cell viability was assessed.A proteomic method,isobaric tags for relative and absolute quantitation (iTRAQ),was employed to detect the proteins altered by β-elemene.Protein expression was validated by Western blot.Results β-elemene inhibited the viability of SGC7901 gastric cancer cells in a dose-dependent manner.Altogether,147 upregulated proteins and 86 downregulated proteins were identified in response to β-elemene treatment in SGC7901 gastric cancer cell line.Among them,the expressions of p21-activated protein kinase-interacting protein 1 (PAK1IP1 ),Bcl-2-associated transcription factor 1 (BTF)and topoisomerase 2-alpha (TOPIIα)were validated by Western blot and the trends were consistent with iTRAQ results.Top pathways involved inβ-elemene treatment in SGC7901 gastric cancer cell line included ribosome signaling,peroxisome proliferator-activated receptors (PPARs)signaling pathway,regulation of actin cytoskeleton,phagosome,biosynthesis and metabolism of some amino acids.Conclusion Our results suggest a promising therapeutic role of β-elemene for gastric cancer.The differentially expressed proteins give us better insights into the potential mechanisms involved in gastric cancer treatment using β-elemene.关键词
β-榄香烯/胃癌/增殖/蛋白质组学/PAK1IP1/TOPIIαKey words
β-elemene/gastric cancer/proliferation/proteomics/PAK1IP1/TOPIIα分类
医药卫生引用本文复制引用
刘俊松,车向明,仇广林,樊林,赵伟,贺仕才,常帅,王曙逢..β-榄香烯对人胃癌细胞 SGC7901作用的蛋白质组学研究[J].西安交通大学学报（医学版）,2015,(6):840-844,861,6.基金项目
国家自然科学基金资助项目(No.81172357) Supported by the National Natural Science Foundation of China (No.81172357) （No.81172357）
