陕西科技大学学报（自然科学版）Issue(6)：116-120,131,6.
酶解桃仁分离蛋白制备肾素和ACE 双重抑制肽的工艺研究
Study on enzymatic hydrolysis conditions for the preparation of dual rennin and ACE inhibitory peptide from peach kernel protein isolate
摘要
Abstract
The preparation of dual rennin and angiotensin‐I‐converting enzyme (ACE) inhibi‐tory peptides from peach kernel protein isolate with Alcalase 2 .4 L was investigated .ACE in‐hibitory rate in vitro and renin inhibitory rate in vitro were investigated with respect to hy‐drolysis parameters including temperature ,pH ,substrate concentration and enzyme dosage . As a result ,the optimum conditions for enzymatic preparation of dual rennin and ACE inhib‐itory peptides from peach kernel protein isolate were found to be hydrolysis at pH 8 .02 ,tem‐perature of 50 .6 ℃ and enzyme dosage of 3 .06% .Under these conditions ,the prepared pep‐tides showed an ACE inhibitory rate of 69 .03% ,renin inhibitory rate of 57 .61% .关键词
桃仁分离蛋白/肾素/ACE抑制肽/响应面法Key words
peach kernel protein isolate/rennin/ACE inhibitory peptide/response surface methodology分类
化学工程引用本文复制引用
朱振宝,杨梅,易建华,王留续..酶解桃仁分离蛋白制备肾素和ACE 双重抑制肽的工艺研究[J].陕西科技大学学报（自然科学版）,2015,(6):116-120,131,6.基金项目
陕西省科技厅农业科技攻关计划项目 （）
