现代妇产科进展2015，Vol.24Issue(11)：801-804,4.DOI:10.13283/j.cnki.xdfckjz.2015.11.001
InsR/FoxO1信号通路对妊娠胆汁淤积症子代大鼠下丘脑中POMC表达的影响
Effect of InsR/FoxO1 on the expression of POMC in the hypothalamus of intrahepatic cholestasis of pregnancy rats offspring
摘要
Abstract
Objective:To study the effect of InsR/FoxO1 on the expression of POMC in the hypothalamus of the intrahepatic cholestasis of pregnancy rats offspring. Methods:40 clean SD pregnant rats were selected and divided into two groups at random,20 in every group. Since the 13th day of pregnancy,Control group was injected subcutaneously with refined vegetable oil 2 . 0 ml/( kg · d ) , ICP group was injected subcutaneously with the 17-α-ethynylestradio ( EE ) 1 . 0 mg/( kg · d ) . 20 Female 0 ffspring in both ICP group and control group were selected at random and feed to six months. At the 24 weeks of age,offspring underwent a glucose tolerance test. All rats were killed at the six months. The mRNA of InsR、FoxO1 and POMC in hypothala-mus were examined by real-time PCR and western blot. The expression of POMC in hypothalam-ic arcuate of 6 months offspring was also examined by immunohistochemistry. Result:The glu-cose values of female in ICP group were higher than that of control group offspring(P<0. 05).The mRNA expressions of InsR、FoxO1 and POMC in hypothalamus in ICP group offspring were different with control offspring at six months ( P<0 . 05 ) . The positive cells of POMC in hypotha-lamic arcuate in ICP group were little than that control group. Conclusion:Bile acid levers is higher in ICP group. The bad intrauterine environment may be a major contributor to change of InsR/FoxO1 and POMC decreased. ICP offspring showed a hunger state and then gained weight.关键词
胆汁淤积/下丘脑/弓状核/胰岛素受体/胰岛素调节转录因子/POMCKey words
Cholestasis of pregnancy/Hypothalamus/Arcuate nucleus/InsR/FoxO1/Pomc分类
医药卫生引用本文复制引用
闫时,王晶晶,宋昭逸,时青云..InsR/FoxO1信号通路对妊娠胆汁淤积症子代大鼠下丘脑中POMC表达的影响[J].现代妇产科进展,2015,24(11):801-804,4.基金项目
国家自然科学基金(No:81170588) （No:81170588）
