中国组织工程研究Issue(1)：17-22,6.DOI:10.3969/j.issn.2095-4344.2013.01.003
体外诱导骨髓间充质干细胞向胆管上皮样细胞分化
In vitro induced differentiation of bone marrow mesenchymal stem cells into bile duct epithelial-like cells
张诚 1杨玉龙 1林美举 1史力军 1张洪威 1李婧伊1
作者信息
- 1. 大连大学附属中山医院胆道微创外科，辽宁省大连市 116001
- 折叠
摘要
Abstract
BACKGROUND:Extrahepatic bile duct and gal bladder epithelial cel s are easily isolated and purified, but bile duct epithelial cel s predispose to lose proliferative capacity under in vitro circumstances. Thus, enough amounts of cel s used for basic studies are hardly acquired, which limits the progression of bile duct repair. Bone marrow mesenchymal stem cel s can be differentiated into hepatocytes, but there have been no reports regarding in vitro induced differentiation of bone marrow mesenchymal stem cel s into bile duct epithelial cel s. OBJECTIVE:To discuss the feasibility of inducing bone marrow mesenchymal stem cel s into the bile duct epithelial cel s in vitro, and to find suitable seed cel s for the repair of bile duct injury. METHODS:Rat bone marrow mesenchymal stem cel s were in vitro isolated and purified by whole bone marrow adherent screening in vitro. Passage 3 bone marrow mesenchymal stem cel s were cultured with culture medium containing hepatocyte growth factor and epidermal growth factor. The morphological changes of bone marrow mesenchymal stem cel s were observed under the inverted microscope and CK19 expression was detected by immunofluorescence assay at different time periods. RESULTS AND CONCLUSION:Under the induction by hepatocyte growth factor and epidermal growth factor, bone marrow mesenchymal stem cel s gradual y appeared from shuttle-shaped to polygonal or triangular. Immunofluorescence examination showed that CK19 expression appeared in the cel membrane in the 4th week of induction and it was significantly increased in the 6th week. These findings suggest that bone marrow mesenchymal stem cel s can be induced to differentiate into duct epithelial-like cel s under the combined induction of hepatocyte growth factor and epidermal growth factor, which provides a new thought for bile duct repair with bone marrow mesenchymal stem cel s.关键词
干细胞/骨髓干细胞/骨髓间充质干细胞/肝细胞生长因子/表皮生长因子/胆管上皮细胞/CK19/干细胞图片文章Key words
stem cells/bone marrow-derived stem cells/bone marrow mesenchymal stem cells/hepatocyte growth factor/epidermal growth factor/bile duct epithelial cells/CK19/stem cel photographs-containing paper分类
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张诚,杨玉龙,林美举,史力军,张洪威,李婧伊..体外诱导骨髓间充质干细胞向胆管上皮样细胞分化[J].中国组织工程研究,2013,(1):17-22,6.
