中国组织工程研究Issue(2)：228-234,7.DOI:10.3969/j.issn.2095-4344.2013.02.008
超脉冲CO2点阵激光干预兔耳浅表性瘢痕的原位再生*
Ultrapulse CO2 fractional laser intervens in situ superficial scar regeneration in a rabbit ear
摘要
Abstract
BACKGROUND:The ultrapulse CO2 fractional laser has a good effect on the treatment of superficial scars, but the mechanism is unclear. OBJECTIVE:To investigate the effect of ultrapulse CO 2 fractional laser on the treatment of superficial scars in rabbit ear and to explore mechanism of wound in situ regeneration and repair. METHODS:The superficial scars in rabbit ear were induced with ultrapulse CO 2 fractional laser, then traditional iodophor treatment and moist exposed burn ointment treatment methods were used for repairing the wound. RESULTS AND CONCLUSION:The wound healing time of moist exposed burn ointment group was shorter than that of iodophor group (P<0.01). The epidermal stem cel s emerged after the superficial scars induced with ultrapulse CO2 fractional laser, and the expression of cytokeratin 19 and P63 protein was observed after induced for 24 hours. The number of cytokeratin 19 and P63 protein in the moist exposed burn ointment was increased after induced for 4 days and reached peak at 7 days and then decreased at 14 days. While the number of cytokeratin 19 and P63 protein in the iodophor group was increased after induced for 4 days, and then decreased after induced for 7 and 14 days. The expression of cytokeratin 19 and P63 protein in the moist exposed burn ointment group was significantly higher than that in the iodophor group (P<0.05). Ultrapulse CO2 combined with moist exposed burn ointment for the treatment of superficial scar in rabbit ear can better activate and protect the epidermal stem cel s, and promote wound repair and in situ regeneration. fractional laser关键词
组织构建/皮肤组织构建/超脉冲CO2激光/点阵激光/浅表性瘢痕/表皮干细胞/细胞角蛋白19/P63蛋白/省级基金/组织构建图片文章Key words
tissue construction/skin tissue construction/ultrapulse CO 2 laser/fractional laser/superficial scar/epidermal stem cel s/cytokeratin 19/P63 protein/provincial grants-supported paper/tissue construction photographs-containing paper分类
医药卫生引用本文复制引用
谭军,雷颖,李高峰,欧阳华伟,朱轶,李波..超脉冲CO2点阵激光干预兔耳浅表性瘢痕的原位再生*[J].中国组织工程研究,2013,(2):228-234,7.基金项目
湖南省科技厅资助项目(2009-JT-3039)。 （2009-JT-3039）
