中国组织工程研究Issue(2)：235-240,6.DOI:10.3969/j.issn.2095-4344.2013.02.009
RhoA/ROCK信号通路在异常应力下关节软骨中的表达★
Variation of RhoA/ROCK transduction pathway in the articular cartilages under abnormal stress
张海翔 1王宸1
作者信息
- 1. 东南大学附属中大医院，江苏省南京市 210009
- 折叠
摘要
Abstract
BACKGROUND:In order to confirm whether RhoA/ROCK transduction pathway play an active role in in vivo articular cartilage degeneration or osteoarthritis, we designed this experiment. OBJECTIVE:To explore the role of the RhoA/ROCK transduction pathway in the process of articular cartilage degeneration. METHODS:Total y 36 New Zealand white rabbits were randomly divided into a control and 2-, 4-and 6-week immobilization groups, and the right hindlimbs of rabbits were immobilized with plaster bandage in extension position except those in the control group. The articular cartilage of the lateral tibial plateau was harvested for hematoxylin-eosin staining and immunohistochemistry to observe the pathological changes and the content of RhoA and ROCK. RESULTS AND CONCLUSION:The articular cartilage degenerated with time prolonged, and the Mankin scores showed there were significant differences among groups (P<0.05). Immunohistochemical results showed that the RhoA/ROCK transduction pathway was motivated mainly in the tangent layer and the middle layer of the cartilage and inhibited with immobilization time prolonged. The RhoA/ROCK transduction pathway takes an important role in the process of articular cartilage degeneration.关键词
组织构建/软骨组织构建/异常应力/关节软骨/RhoA/ROCK/免疫组织化学/制动/组织构建图片文章Key words
tissue construction/cartilage tissue construction/abnormal stress/articular cartilage/RhoA/ROCK/immunohistochemistry/immobilization/tissue construction photographs-containing paper分类
医药卫生引用本文复制引用
张海翔,王宸..RhoA/ROCK信号通路在异常应力下关节软骨中的表达★[J].中国组织工程研究,2013,(2):235-240,6.
