中国组织工程研究Issue(2)：247-253,7.DOI:10.3969/j.issn.2095-4344.2013.02.011
碘乙酸钠诱导原代大鼠软骨细胞凋亡★
Monosodium iodoacetate induces apoptosis of primary rat chondrocytes
姜丽平 1李龙婕 2宫德正 1耿成燕 1姜丽杰 3仲来福1
作者信息
- 1. 大连医科大学-中日合作医药科学研究所，辽宁省大连市 116044
- 2. 大连医科大学附属第一医院放疗科，辽宁省大连市 116023
- 3. 大连大学附属中山医院内科，辽宁省大连市 116001
- 折叠
摘要
Abstract
BACKGROUND:Recently, monosodium iodoacetate is usual y used to cause cartilage degradation resembling the pathological changes of human osteoarthritis. However, rate studies report the mechanisms underlying monosodium iodoacetate-induced chondrocyte apoptosis. OBJECTIVE:To assess the apoptosis of primary rat chondrocytes induced by different concentrations of monosodium iodoacetate and to clarify the underlying mechanism. METHODS:The primary rat chondrocytes were treated with monosodium iodoacetate for 24 hours, and the induction of apoptosis was analyzed by flow cytometry and Hoechst 33342/PI staining. The levels of mitochondrial membrane potential were evaluated using fluorescence spectrophotometer and laser scanning confocal microscope. The production of reactive oxygen species was determined by fluorescence spectrophotometer. Apoptosis-related protein cytochrome C and caspase-3 expressions were examined by western blotting. RESULTS AND CONCLUSION:Monosodium iodoacetate induced cel ular apoptosis in a dose-dependent manner. Significantly increased level of reactive oxygen species was observed in primary rat chondrocytes at higher concentration of monosodium iodoacetate (P<0.05). Significantly decreased levels of mitochondrial membrane potential were also shown in primary rat chondrocytes (P<0.05). Western blot assay revealed that monosodium iodoacetate significantly increased the release of cytochrome C and the protein expression of caspase-3, leading to cel apoptosis. Together these observations suggest that the mechanisms of monosodium iodoacetate-induced apoptosis are primarily via the production of reactive oxygen species and mitochondria-mediated caspase-3 activation in primary rat chondrocytes.关键词
组织构建/软骨组织构建/软骨细胞/碘乙酸钠/活性氧/线粒体膜电位/细胞色素C/Caspase-3/凋亡/组织构建图片文章Key words
tissue construction/cartilage tissue construction/chondrocytes/monosodium iodoacetate/reactive oxygen species/mitochondrial membrane potential/cytochrome C/Caspase-3/apoptosis分类
医药卫生引用本文复制引用
姜丽平,李龙婕,宫德正,耿成燕,姜丽杰,仲来福..碘乙酸钠诱导原代大鼠软骨细胞凋亡★[J].中国组织工程研究,2013,(2):247-253,7.
