中国组织工程研究Issue(2)：280-285,6.DOI:10.3969/j.issn.2095-4344.2013.02.017
膝关节后交叉韧带断裂对内侧副韧带组织学的影响☆
Effect of posterior cruciate ligament rupture on histological features of medial collateral ligament
俞芳 1李康华 1章灿 1李宇晟 1高曙光 1卢邦宝1
作者信息
- 1. 中南大学湘雅医院骨科研究所，中南大学湘雅医院骨科，湖南省长沙市 410008
- 折叠
摘要
Abstract
BACKGROUND: By comparing the relative quantity of typeⅠ and III col agens and type Ⅰ/Ⅲ col agen ratio, we can determine the ligament histology performance to some extent. OBJECTIVE:To explore the histological effect of posterior cruciate ligament injury on the medial col ateral ligament under a lower physiological load in rabbits. METHODS:Bilateral knees of 24 male rabbits were enrol ed for a self-control trial. Posterior cruciate ligaments at the experimental side were transected, and those in the control side were only exposed but not transected. At the 8th, 16th, 24th and 40th weeks after modeling, six rabbits were executed randomly, and then the histological alteration of the medial col ateral ligament was observed through hematoxylin-eosin staining and picrosirius-polarization staining. RESULTS AND CONCLUSION:(1)Hematoxylin-eosin staining:At the 8 th, 16thand 24th weeks, there was no obvious difference in the col agen distribution and arrangement of the medial col ateral ligament between experimental group and control group;at the 40th week, the col agen was thinner in the experimental group. (2)Picrosirius-polarization staining:At the 8th, 16th and 24th weeks, the total number of type Ⅰ and Ⅲ col agens in the medial col ateral ligament of the experiment group was more than that in the control group (P<0.05);however, at the 40th week, the total number of type I and III col agens in the medial col ateral ligament of the experimental group was much fewer than that of the control group (P<0.05);at the 8th week, ratio of type Ⅰ/Ⅲcol agen in the medial col ateral ligament of the experimental group did not differ from that in the control group (P>0.05);however, at the 16th, 24th and 40th weeks, the ratio of type Ⅰ/Ⅲ col agen in the experimental group was much fewer than that in the control group (P<0.05). In short-term, posterior cruciate ligament injury has no significant effect on histological properties of the rabbit medial col ateral ligament. However, the histological properties can decrease significantly with time.关键词
组织构建/组织构建与生物力学/膝关节/后交叉韧带/断裂/内侧副韧带/胶原纤维/组织学特征/韧带修复/组织构建图片文章Key words
tissue construction/tissue construction and biomechanics/knee/posterior cruciate ligament/fracture/medial coll ateral ligament/coll agen fibers/histological features/ligament repair/tissue construction photographs-containing paper分类
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俞芳,李康华,章灿,李宇晟,高曙光,卢邦宝..膝关节后交叉韧带断裂对内侧副韧带组织学的影响☆[J].中国组织工程研究,2013,(2):280-285,6.
