中国组织工程研究Issue(2)：286-290,5.DOI:10.3969/j.issn.2095-4344.2013.02.018
尿酸钠结晶影响肾小管上皮细胞的紧密连接★
Monosodium urate monohydrate crystals affect the tight junctions among renal tubular epithelial cells
李慧娟 1陈敢 1傅君舟 1周姗姗1
作者信息
- 1. 广州医学院附属广州市第一人民医院，广东省广州市 510182
- 折叠
摘要
Abstract
BACKGROUND:Up to now, the relationship of uric acid stones with tight junction proteins and renal interstitial fibrosis is yet unclear. OBJECTIVE:To observe the effect of monosodium urate (MSU) monohydrate crystals on the tight junction among NRK-52E cel s. METHODS:The NRK-52E cel s were divided into two groups:control group and MSU group cultured in serum-free medium and MSU crystals, respectively. The protein and mRNA expressions of ZO-1 were investigated by immunofluorescence and reverse transcription-PCR, respectively. RESULTS AND CONCLUSION:Compared with the control group, the protein and mRNA expressions of ZO-1 were down-regulated after the stimulation of MSU at 24, 48, 72 hours (P<0.05), especial y at 72 hours (P<0.01). Protein expression appeared with redistribution phenomenon. These findings indicate that MSU can damage the structure, function and distribution of tight junction proteins of renal tubular epithelial cel s.关键词
组织构建/组织构建细胞学实验/尿酸钠结晶/紧密连接/紧密连接蛋白/肾小管上皮细胞/NRK-52E细胞/肾间质纤维化/高尿酸血症/跨膜蛋白/膜周蛋白/大鼠/组织构建图片文章分类
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李慧娟,陈敢,傅君舟,周姗姗..尿酸钠结晶影响肾小管上皮细胞的紧密连接★[J].中国组织工程研究,2013,(2):286-290,5.
