中国组织工程研究Issue(3)：445-451,7.DOI:10.3969/j.issn.2095-4344.2013.03.011
复合水凝胶人工角膜裙边支架材料的生物相容性评价*
Biocompatibility of a hydrogel skirt scaffold in artificial cornea
摘要
Abstract
BACKGROUND: Beta-tricalcium phosphate/poly (vinyl alcohol) hydrogel (β-TCP/PVA-H) composite is a material with high water content, good softness, which is beneficial to the fibroblasts growth and col agen deposition. OBJECTIVE: To study the biocompatibility of β-TCP/PVA-H composite as an artificial cornea skirt material. METHODS: Delayed-type hypersensitivity: Both sides of the spinal skin of the guinea pig from beginning to end were injected with A solution (the mixture of complete Freund’s adjuvant and normal saline), B solution (extraction ofβ-TCP/PVA-H composite or normal saline or 2-Mercaptobenzothiazole), C solution (mixture of A and B solutions at equal volume), fol owed by local induction and inspire. The acute systemic toxicity test: Kunming mouse tail vein was injected with normal saline and β-TCP/PVA-H composite extraction. In vitro cytotoxicity assays: MRC-5 cells were cultured by β-TCP/PVA-H composite extraction, cel culture medium and phenol-containing cel culture medium. Intradermal reaction: The extracts of β-TCP/PVA-H composite, normal saline was injected intradermal y into the rabbit spine by normal saline (or sesame oil), normal saline and sesame oil. RESULTS AND CONCLUSION: The biocompatibility experiments of β-TCP/PVA-H composite showed that delayed-type hypersensitivity was graded 0-1, acute systemic toxicity test showed no symptoms, in vitro cytotoxicity was graded 0 or 1, and skin irritations were very slight. The experiments are agreed with the relative national standards, and β-TCP/PVA-H composite skirt as artificial cornea scaffold is a qualified medical material.关键词
生物材料/材料生物相容性/β-磷酸三钙/聚乙烯醇/水凝胶/人工角膜/体外细胞毒性/急性全身毒性/皮内反应/迟发型超敏反应/其他基金Key words
biomaterials/material biocompatibility/beta-tricalcium phosphate/poly (vinyl alcohol)/hydrogel/artificial cornea/in vitro cytotoxicity/acute systemic toxicity/intradermal reaction/delayed-type hypersensitivity/other grants-supported paper分类
医药卫生引用本文复制引用
邱岗峰,石春芝,周金生,麦卫馨,欧阳君君,吴三强,丘渭遥..复合水凝胶人工角膜裙边支架材料的生物相容性评价*[J].中国组织工程研究,2013,(3):445-451,7.基金项目
深圳大学科技计划项目(20074SCZ)。Supported by：the Science and Technology Plan of Shenzhen University, No.20074SCZ* （20074SCZ）
