中国组织工程研究Issue(3)：457-464,8.DOI:10.3969/j.issn.2095-4344.2013.03.013
许旺细胞及小肠黏膜下层复合生长因子缓释微球修复周围神经缺损*☆
Combination of Schwann cells, small intestinal submucosa and growth factor sustained-release microspheres for repair of peripheral nerve defects
摘要
Abstract
BACKGROUND: To combine Schwann cells (SCs) and smal intestinal submucosa (SIS) with basic fibroblast growth factor (bFGF) is a feasible way to construct artificial nerve that possesses nerve activity and chemotaxis in vitro. OBJECTIVE: To observe the recanalization of neural pathways after repairing peripheral defects with bFGF control ed release microspheres combined with SCs and SIS. METHODS: Models of sciatic nerve defects were established in Sprague-Dawley rats, and then randomized into:experimental group repaired with bFGF control ed release microspheres combined with SCs and SIS, positive control group with SCs and SIS combined with free bFGF, negative control group with SCs and SIS, and blank control group with autologous nerve. RESULTS AND CONCLUSION: The number of regenerated nerve fibers, number of DiI tracer-positive neurons, positive expression rate of S-100 and neurofilament protein, ultrastructure of regenerated myelin and axons, nerve conduction velocity and composite action potential in the experimental group were superior to those in the positive and negative control groups at 16 weeks after operation (P < 0.05). These findings indicate that bFGF control ed release microspheres combined with SCs and SIS can reconstruct the neural pathways fol owing sciatic nerve injury.关键词
生物材料/生物材料与药物控释/碱性成纤维细胞生长因子/小肠黏膜下层/许旺细胞/周围神经缺损/神经传导/缓释微球/省级基金/生物材料图片文章分类
医药卫生引用本文复制引用
张开伟,段宏,项舟,肖睿,陈久毅..许旺细胞及小肠黏膜下层复合生长因子缓释微球修复周围神经缺损*☆[J].中国组织工程研究,2013,(3):457-464,8.基金项目
四川省宜宾市重点科技计划项目(200803010)。Supported by：the Key Scientific Plan of Yibin City, No.200803010* （200803010）
