中国组织工程研究Issue(5)：866-871,6.DOI:10.3969/j.issn.2095-4344.2013.05.016
移植异体血管内皮祖细胞向大鼠损伤皮肤的趋化**☆
Chemotaxis of vascular endothelial progenitor cells towards rat skin wounds following allogeneic transplantation
摘要
Abstract
BACKGROUND: It becomes a hotspot that al ogeneic endothelial progenitor cells participate in vascular remodeling in ischemic tissues of the body. OBJECTIVE: To observe the chemotaxis of al ogeneic transplantation of vascular endothelial progenitor cells to rat skin wounds. METHODS: Endothelial progenitor cells isolated from the peripheral blood of rats were cultured and labeled with BrdU, and then injected into rats with cutting injury on the back skin via the tail vein. Another normal rats served as controls. The number of BrdU positive endothelial progenitor cells in skin wound areas was detected by immunofluorescence staining at 1, 3, 7, 14 days after transplantation, and the changes in the number and volume of spleen lymphoid fol icles were observed by hematoxylin-eosin staining. RESULTS AND CONCLUSION: Endothelial progenitor cells could move to the injured skin areas. The number of endothelial progenitor cells was most at 3 and 7 days (P < 0.05), and BrdU positive cells could be seen until the 14th day in the vessel wal of the injured skin area. There was no significant difference between the two groups in the number and volume of spleen lymphoid fol icles. No significant immunological rejection was caused by the al ogeneic transplantation of vascular endothelial progenitor cells.关键词
器官移植/组织移植/血管内皮祖细胞/移植/皮肤损伤/大鼠脾脏/免疫排斥/异体移植/其他基金/器官移植图片文章Key words
Organ transplantation/tissue transplantation/vascular endothelial progenitor cells/transplantation/skin injury/rat spleen/immune rejection/allogeneic transplantation/other grants-supported paper分类
医药卫生引用本文复制引用
刘超,赵计林,苗雷英..移植异体血管内皮祖细胞向大鼠损伤皮肤的趋化**☆[J].中国组织工程研究,2013,(5):866-871,6.基金项目
南京市青年启动基金(2011-19-198)；南京市卫生青年人才培养第三层次基金。Supported by：the Youth Start Fund of Nanjing City, No.2011-19-198* （2011-19-198）
the Third Level Fund for the Young Talents in the Health Field of Nanjing City* （）
