中国组织工程研究Issue(5)：878-885,8.DOI:10.3969/j.issn.2095-4344.2013.05.018
脂肪干细胞介导的自体脂肪移植隆胸★
Auto-adipose stem cells facilitate autologous fat transfer for breast augmentation
闫俊灵 1狄国虎 2丁红 1张巍 1刘嘉琦 1汤苏阳1
作者信息
- 1. 武装警察部队总医院皮肤再生医学科，北京市100039
- 2. 辽宁医学院，辽宁省锦州市121000
- 折叠
摘要
Abstract
BACKGROUND: Human adipose stem cells have the capability of multipotential differentiation and have a promising prospect in the field of tissue engineering and regenerative medicine. OBJECTIVE: To evaluate the technology and efficacy of human adipose stem cells in autologous fat grafting breast augmentation. METHODS: Fifteen patients with auto-adipose injection breast augmentation were fol owed-up for 3-6 months. The whole operation was separated into two phases, in the first phase, 15-20 mL adipose tissue was aspirated and digested with col agenase Ⅰ to separate and amplify the human adipose stem cells. When cultured to passage 2, the plastic adherent stem cells were up to 1×108 and verificated by immunophenotype, differentiation and genetic stability assays before transplantation. In the second phase, 300-450 mL adipose tissue was aspirated and transplanted combined with human adipose stem cells. RESULTS AND CONCLUSION: The passage 2 human adipose stem cells exhibited typical mesenchymal stem cells characteristics and maintained genetic stability. The 3-6 months fol ow-up demonstrated that there was no apparent adipose absorption, infection, pain and upper limb activity limitation, and the effect was good. The authors suggested that autologous adipose derived stem cells-mediated auto-adipose injection breast augmentation is a practical and efficacious method.关键词
器官移植/组织移植/组织细胞移植/脂肪干细胞/间充质干细胞/遗传稳定性/免疫表型/分化潜能/隆胸/自体脂肪颗粒注射/器官移植细胞图片文章分类
医药卫生引用本文复制引用
闫俊灵,狄国虎,丁红,张巍,刘嘉琦,汤苏阳..脂肪干细胞介导的自体脂肪移植隆胸★[J].中国组织工程研究,2013,(5):878-885,8.
