中国组织工程研究Issue(6)：951-956,6.DOI:10.3969/j.issn.2095-4344.2013.06.001
β射线照射皮肤损伤创面注射骨髓间充质干细胞后的创面愈合*☆
Bone marrow mesenchymal stem cell injection for treatment of β-ray-irradiated skin injury in rats
摘要
Abstract
BACKGROUND: The β-ray-irradiated skin injury is difficult to heal and there is no effective treatment method for β-ray-irradiated skin injury. OBJECTIVE: To investigate the effect of bone marrow mesenchymal stem cel transplantation in treatment of β-ray-irradiated skin injury in rats. METHODS: Three Sprague-Dawley female rats were kil ed to isolate and culture the bone marrow mesenchymal stem cells, then the cells were labeled with 4',6-diamidino-2-phenylindole to prepare the <br> bone marrow mesenchymal stem cells suspension after passaged to the fifth generation. Forty-seven Sprague-Dawley female rats of 3 months old and clean grade were randomly divided into three groups: treatment group, control group, and normal group. In the treatment group, single dosage (45 Gy) of β-ray irradiation produced by linear accelerator was applied on buttock skin (40 mm×30 mm) in rats, and the acute deep injury model was established. Bone marrow mesenchymal stem cellsuspension was injected in the subcutaneous and dermal layers after the wound appeared. The rats in the control group were irradiated as those in the treatment group, and placebo was injected when the wound appeared, with the same method as the treatment group. Rats in the normal group were not irradiated. The pathological changes of wound tissue of rats in each group were observed at 1, 2, 3 and 4 weeks after treatment under light microscope, and the concentration of CD31, CK4 and fibroblast growth factor was detected by immunohistochemistry. RESULTS AND CONCLUSION: The wound healing time in the treatment group was shorter than that in the control group (P < 0.05). Immunohistochemistry showed the number of CD31, CK4 and fibroblast growth factor positive cells in the treatment group at 1-4 weeks after bone mesenchymal stem cellsuspension injection was significantly greater than that in the control group (P < 0.05). It indicates that bone mesenchymal stem cel injection can promote the wound healing of β-ray-irradiated skin injury and reduce wound healing time.关键词
干细胞/骨髓干细胞/骨髓间充质干细胞/β射线/皮肤损伤/创面/创面愈合/大鼠/成纤维细胞/组织再生/组织工程/其他基金/干细胞图片文章Key words
stem cells/bone marrow-derived stem cells/bone marrow mesenchymal stem cells/β ray/skin injury/wound/wound healing/rats/fibroblasts/tissue regeneration/tissue engineering/other grants-supported paper/stem cel photographs-containing paper分类
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沈国良,苏本玄,林伟,祁强,赵小瑜,陆兴安..β射线照射皮肤损伤创面注射骨髓间充质干细胞后的创面愈合*☆[J].中国组织工程研究,2013,(6):951-956,6.基金项目
苏州市科技发展计划(应用基础研究)基金资助项目(SYS201019)。Supported by：Suzhou Science Development Planning Project, No. SYS201019* （应用基础研究）
