中国组织工程研究Issue(6)：1029-1036,8.DOI:10.3969/j.issn.2095-4344.2013.06.014
人脐血间充质干细胞尾静脉移植治疗扩张型心肌病★
Tail vein transplantation of human umbilical cord blood mesenchymal stem cells for treatment of dilated cardiomyopathy
罗建红 1何学华 1廖金卯 1袁勇华 1胡沙雅1
作者信息
- 1. 湖南师范大学第一附属医院，湖南省长沙市410000
- 折叠
摘要
Abstract
BACKGROUND: In vitro studies have demonstrated that human umbilical cord blood mesenchymal stem cells can differentiate into autorhythmic myocardial cells. Few studies are reported regarding tail vein transplantation of human umbilical cord blood mesenchymal stem cells for treatment of dilated cardiomyopathy. OBJECTIVE: To investigate the influences of tail vein transplantation of human umbilical cord blood mesenchymal stem cells on myocardial structure and heart function in a rat model of dilated cardiomyopathy. METHODS: Dilated cardiomyopathy was induced in Wistar rats by intraperitoneal injection of adriamycin. In the dilated cardiomyopathy group, human umbilical cord blood mesenchymal stem cells were injected into the rats via the tail vein at 8 weeks after dilated cardiomyopathy induction. In the model control group, equal amounts of DMEM were identical y injected. In the blank control group, the rats were not subjected to dilated cardiomyopathy induction, but the same amount of physiological saline was injected at the same time point. RESULTS AND CONCLUSION: Compared with the blank control group, the heart function of rats in the dilated cardiomyopathy group and model control group was significantly injured, and the disease severity was milder in the dilated cardiomyopathy group than in the model control group. Troponin T expression was detected in the cells of rats receiving tail transplantation of human umbilical cord blood mesenchymal stem cells. These findings suggest that human umbilical cord blood mesenchymal stem cells can promote the recovery of heart function and al eviate the lesion of myocardial tissue in rats with dilated cardiomyopathy.关键词
干细胞/干细胞移植/脐血间充质干细胞/细胞培养/细胞移植/尾静脉/扩张型心肌病/心功能/心肌结构/阿霉素/干细胞图片文章Key words
stem cells/stem cel transplantation/umbilical cord blood mesenchymal stem cells/cel culture/cel transplantation/tail vein/dilated cardiomyopathy/heart function/myocardial structure/adriamycin/stem cel photographs-containing paper分类
医药卫生引用本文复制引用
罗建红,何学华,廖金卯,袁勇华,胡沙雅..人脐血间充质干细胞尾静脉移植治疗扩张型心肌病★[J].中国组织工程研究,2013,(6):1029-1036,8.
