中国组织工程研究Issue(6)：1049-1055,7.DOI:10.3969/j.issn.2095-4344.2013.06.017
骨髓间充质干细胞移植治疗矽肺小鼠肺炎及肺纤维化*★
Transplantation of bone marrow mesenchymal stem cells for treatment of pneumonitis and pulmonary fibrosis in silicosis mice
摘要
Abstract
BACKGROUND: Silicosis is a serious disease that harms human’s health. So far, there has been no effective treatment strategy. OBJECTIVE: To investigate the therapeutic effects of bone marrow mesenchymal stem cel transplantation on silicosis. METHODS: Thirty-six C57BL/6 mice were randomly divided into three groups. In the control group, mice received intrateacheal injection of physiological saline. Mice from the silicosis model group and bone marrow mesenchymal stem cel transplantation group received intrateacheal injection of silica crystals (SiO2) to establish silicosis mouse models. In the bone marrow mesenchymal stem cel transplantation group, bone marrow mesenchymal stem cells were transfused into the tail vein at 6 hours after silicosis induction. RESULTS AND CONCLUSION: Hydroxyproline content in lung parenchyma in the silicosis model group and bone marrow mesenchymal stem cel transplantation group were significantly higher compared to the control group (P <0.01). The hydrocyproline content in the bone marrow mesenchymal stem cel transplantation group was significantly higher than that in the silicosis model group (P < 0.01). The lung index in the silicosis model group and bone marrow mesenchymal stem cel transplantation group was significantly higher than that in the control group (P < 0.01), but the lung index was significantly lower in the bone marrow mesenchymal stem cel transplantation group than in the silicosis model group (P < 0.01). Interleukin-1β expression in the lung tissue was significantly higher in the silicosis model group and bone marrow mesenchymal stem cel transplantation group than that in the control group (P < 0.01), and interleukin-1β expression in the bone marrow mesenchymal stem cel transplantation group was significantly lower than that in the silicosis model group (P < 0.01). Transforming growth factor β1 expression in the lung tissue was significantly higher in the silicosis model group (P < 0.01) and bone marrow mesenchymal stem cel transplantation group (P < 0.05) than that in the control group, and transforming growth factor β1 expression was significantly lower in the bone marrow mesenchymal stem cel transplantation group was significantly lower than that in the silicosis model group (P < 0.01). These findings suggest that bone marrow mesenchymal stem cel transplantation can al eviate pulmonary inflammatory reaction and pulmonary fibrosis.关键词
干细胞/干细胞移植/骨髓间充质干细胞/移植/矽肺/肺炎/肺纤维化/小鼠/绿色荧光蛋白/二氧化硅/白细胞介素/转化生长因子/羟脯氨酸/其他基金/干细胞图片文章分类
医药卫生引用本文复制引用
潘雍,杨昆,刘永哲..骨髓间充质干细胞移植治疗矽肺小鼠肺炎及肺纤维化*★[J].中国组织工程研究,2013,(6):1049-1055,7.基金项目
天津医科大学校局级基金(2011KY40)：尾静脉回输骨髓间充质干细胞对小鼠矽肺纤维化的抑制作用及其机制的研究。Supported by：Bureau-level Foundation of Tianjin Medical University, No.2011KY40* （2011KY40）
