中国组织工程研究Issue(6)：1094-1100,7.DOI:10.3969/j.issn.2095-4344.2013.06.024
益气活血类药物血清对嗅鞘细胞增殖及相关因子分泌的影响☆
Serum-containing drugs responsible for supplementing qi and activating blood circulation influences proliferation of olfactory ensheathing cells and secretion of related cytokines
饶耀剑 1朱文潇 1马文虎 1王拥军2
作者信息
- 1. 河南省洛阳正骨医院脊椎外三科，河南省洛阳市471000
- 2. 上海中医药大学附属龙华医院，上海市200000
- 折叠
摘要
Abstract
BACKGROUND: Supplementing qi and removing blood stasis is the main therapeutic principle of traditional Chinese medicine for the treatment of spinal cord injury, but the efficacy is not plausible. But, whether the application of serum-containing drugs responsible for supplementing qi and activating blood circulation under the guidance of modern technology can treat spinal cord injury and improve the efficacy? OBJECTIVE: To study the effect of serum-containing drugs responsible for supplementing qi and activating blood circulation on the proliferation of olfactory ensheathing cells and the secretion of nerve growth factor and brain-derived neurotrophic factor. METHODS: The olfactory ensheathing cells were in vitro cultured with tissue block culture method. The effect of Buyanghuanwu decoction and Weizheng decoction on the proliferation of olfactory ensheathing cells was detected with MTT assay. The expression levels of nerve growth factor and brain-derived neurotrophic factor in the culture supernatant were detected by western blot assay. RESULTS AND CONCLUSION: MTT results showed that both Buyanghuanwu decoction and Weizheng decoction could promote the proliferation of olfactory ensheathing cells, and there was no significant difference in proliferation-promoting effect. Western blot assay results showed that both Buyanghuanwu decoction and Weizheng decoction could promote the expression of nerve growth factor and brain-derived neurotrophic factor in olfactory ensheathing cells, and there was no significant difference in secretion level between two groups.关键词
干细胞/干细胞与中医药/补阳还五汤/痿证方/益气活血/含药血清/嗅鞘细胞/增殖/神经生长因子/脑源性神经营养因子/促增殖/组织修复/干细胞图片文章分类
医药卫生引用本文复制引用
饶耀剑,朱文潇,马文虎,王拥军..益气活血类药物血清对嗅鞘细胞增殖及相关因子分泌的影响☆[J].中国组织工程研究,2013,(6):1094-1100,7.
