中国组织工程研究Issue(7)：1174-1179,6.DOI:10.3969/j.issn.2095-4344.2013.07.007
原代培养人增生性瘢痕成纤维细胞的生物学行为★
Primary culture of human hypertrophic scar fibroblasts and its biological behavior
杜启翠 1肖文林 2孙桂兰 2马秀兴 1姚如永3
作者信息
- 1. 青岛大学医学院，山东省青岛市266000
- 2. 青岛大学医学院附属医院口腔科，山东省青岛市266000
- 3. 青岛大学医学院附属医院中心实验室，山东省青岛市266000
- 折叠
摘要
Abstract
BACKGROUND: Hypertrophic scar is inevitable in the process of wound healing, but the hypertrophic scar tissue can result in many adverse effects. OBJECTIVE: To establish a reliable method to culture human hypertrophic scar fibroblasts in vitro. METHODS: Human hypertrophic scar fibroblasts were cultured primarily in Dulbecco’s modified Eagle’s medium containing 10% fetal bovine serum using tissue adherent method and digestion method. Fibroblasts were incubated at 37℃in a humidified atmosphere of 5% carbon dioxide. Cel ular morphologies were observed, the growth curve was drawn, and vimentin expression wass detected. RESULTS AND CONCLUSION: (1) After cultured with tissue adherent method, human hypertrophic scar fibroblasts were cultured successful y, first passaged within 20-40 days and then continued to passage every 7-10 days. The cells were long spindle-shaped, and vimentin was positive. (2) Human hypertrophic scar fibroblasts, cultured using digestion method, were cultured successful y and confluent at 15-20 days. The cells were long spindle-shaped, and vimentin was positive. These data have confirmed that both tissue adherent method and digestion method are successful in the primary culture of human hypertrophic scar fibroblasts.关键词
组织构建/皮肤组织构建/人增生性瘢痕/成纤维细胞/原代培养/细胞培养/贴壁法/消化法/波形蛋白/生物学行为/细胞分裂/分裂指数/组织构建图片文章Key words
tissue construction/skin tissue construction/human hypertrophic scar/fibroblasts/primary culture/cel culture/adherent method/digestion/vimentin/biological behavior/cel division/mitotic index/tissue construction photographs-containing paper分类
医药卫生引用本文复制引用
杜启翠,肖文林,孙桂兰,马秀兴,姚如永..原代培养人增生性瘢痕成纤维细胞的生物学行为★[J].中国组织工程研究,2013,(7):1174-1179,6.
