中国组织工程研究Issue(7)：1189-1195,7.DOI:10.3969/j.issn.2095-4344.2013.07.010
激素性股骨头坏死模型中凋亡相关因子的表达★
Apoptosis-related factors in a model of steroid-induced necrosis of the femoral head
张锐东 1张澜 1毛洪刚 1刘国栋1
作者信息
- 1. 内蒙古医科大学第三附属医院骨科，内蒙古自治区包头市014000
- 折叠
摘要
Abstract
BACKGROUND: Recent studies have shown that a large amount of apoptotic osteocytes in the model of steroid-induced necrosis of the femoral head. Caspase-3 and Bcl-2 are two important factors for regulation of cel apoptosis. OBJECTIVE: To observe Bcl-2 and Caspase-3 levels in a rat model of femoral head necrosis and their correlation. METHODS: Sixty healthy Wistar rats were randomly divided into two groups (n=30). Experimental group was treated with gluteal muscle injection of prednisolone 12.5 mg/kg, twice per week, for establishing a femoral head necrosis model; control group was injected with the same amount of normal saline. Rat femoral head was removed after 4, 8 and 12 weeks respectively. Bone tissue morphological changes were observed by hematoxylin-eosin staining and the protein expression levels of Bcl-2 and Caspase-3 were observed using immunohistochemical staining. Linear correlation analysis was performed for analysis of correlation between Bcl-2 and Caspase-3. RESULTS AND CONCLUSION: Femoral heads were complete in the experimental and control groups with smooth and flat cartilage surface. In the experimental group, the trabecular bone in the femoral head gradual y became thinner, the number of adipose cells increased and percentage of empty lacunae significantly increased with the time of administration. Caspase-3 expression increased significantly in the experimental group compared with the control group (P < 0.01), trending to gradual y increase with the time of administration. Conversely, the expression of Bcl-2 was lower in the experimental group than the control group (P < 0.01). The linear correlation analysis showed that there was no correlation between Bcl-2 and Caspase-3. These findings indicate that steroids can increase the expression of Caspase-3 promoting apoptosis of osteocytes and reduce the expression of Bcl-2 inhibiting anti-apoptotic role, which leads to the occurrence and development of steroid-induced necrosis of the femoral head.关键词
组织构建/组织构建实验造模/激素性股骨头坏死/Bcl-2/Caspase-3/相关分析/细胞凋亡/股骨头/骨细胞/组织构建图片文章Key words
tissue construction/experimental modeling in tissue construction/seroid-induced necrosis of the femoral head/Bcl-2/Caspase-3/correlation analysis/apoptosis/femoral head/osteocytes/tissue construction photographs-containing paper分类
医药卫生引用本文复制引用
张锐东,张澜,毛洪刚,刘国栋..激素性股骨头坏死模型中凋亡相关因子的表达★[J].中国组织工程研究,2013,(7):1189-1195,7.
